Ribosomal DNA polymorphism in the genus Bifidobacterium

被引：16

作者：

Mangin, I ^{[1
]}

Bourget, N ^{[1
]}

Decaris, B ^{[1
]}

机构：

[1] UNIV NANCY 1,INRA,ASSOCIATED UNIT 952,FAC SCI,LAB GENET MICROBIOL,F-54506 VANDOEUVRE NANCY,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1996年 / 147卷 / 03期

关键词：

rDNA; Bifidobacterium; identification; RFLP; ribosomal patterns; polymorphism;

D O I：

10.1016/0923-2508(96)80218-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ribosomal DNA polymorphism was studied in order to demonstrate intra- and interspecies differentiation of 42 Bifidobacterium strains. DNA from these strains was digested with the endonucleases BamHI, EcoRV, HindIII and PvuII and then analysed by Southern blotting. Ribosomal patterns using a part of an rRNA 23S gene as a probe dearly differentiated the majority of species from each other. Only B. indicum ATCC 25912(T) and B, infantis ATCC 15697(T) displayed identical ribosomal patterns, even though they are classified into two different species. Moreover, ribotypes were able to distinguish between strains belonging to the same species. Furthermore, these strains generally showed common bands, except for B. infantis strains and two strains of B. animalis.

引用

页码：183 / 192

页数：10

共 20 条

[1] ELECTROPHORETIC PATTERNS OF PROTEINS IN THE GENUS BIFIDOBACTERIUM AND PROPOSAL OF 4 NEW SPECIES [J].