Radioprotection by two phenolic compounds: Chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro

被引：108

作者：

Cinkilic, Nilufer ^{[1
]}

Cetintas, Sibel Kahraman ^{[2
]}

Zorlu, Tolga ^{[3
]}

Vatan, Ozgur ^{[1
]}

Yilmaz, Dilek ^{[1
]}

Cavas, Tolga ^{[1
]}

Tunc, Sema ^{[2
]}

Ozkan, Lutfi ^{[2
]}

Bilaloglu, Rahmi ^{[1
]}

机构：

[1] Uludag Univ, Fac Arts & Sci, Dept Biol, Cell Culture & Genet Toxicol Lab, TR-16059 Bursa, Turkey

[2] Uludag Univ, Fac Med, Dept Radiat Oncol, TR-16059 Bursa, Turkey

[3] Istanbul Univ, Inst Forens Sci & Legal Med, Istanbul, Turkey

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 53卷

关键词：

Radioprotection; Chlorogenic acid; Quinic acid; X-ray irradiation; Comet assay; DNA damage; CULTURED HUMAN-LYMPHOCYTES; GAMMA-RADIATION; CAFFEIC ACID; LIPID-PEROXIDATION; ANTIOXIDANT ACTIVITIES; DERIVATIVES; CELLS; CARCINOGENESIS; FLAVONOIDS; MECHANISM;

D O I：

10.1016/j.fct.2012.12.008

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The present study was designed to determine the radioprotective effect of two phytochemicals, namely, quinic acid and chlorogenic acid, against X-ray irradiation-induced genomic instability in non-tumorigenic human blood lymphocytes. The protective ability of two phenolic acids against radiation-induced DNA damage was assessed using the alkaline comet assay in human blood lymphocytes isolated from two healthy human donors. A Siemens Mevatron MD2 (Siemens AG, USA, 1994) linear accelerator was used for irradiation. The results of the alkaline comet assay revealed that quinic acid and chlorogenic acid decreased the DNA damage induced by X-ray irradiation and provided a significant radioprotective effect. Quinic acid decreased the presence of irradiation-induced DNA damage by 5.99-53.57% and chlorogenic acid by 4.49-48.15%, as determined by the alkaline comet assay. The results show that quinic acid and chlorogenic acid may act as radioprotective compounds. Future studies should focus on determining the mechanism by which these phenolic acids provide radioprotection. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：359 / 363

页数：5

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