MELTING, computing the melting temperature of nucleic acid duplex

被引：93

作者：

Le Novère, N ^{[1
]}

机构：

[1] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England

来源：

BIOINFORMATICS | 2001年 / 17卷 / 12期

关键词：

D O I：

10.1093/bioinformatics/17.12.1226

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix-coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. Perl scripts are provided to show how MELTING can be used to construct more ambitious programs.

引用

页码：1226 / 1227

页数：2

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