Leptin secretion from adipose tissue in women - Relationship to plasma levels and gene expression

被引:175
作者
Lonnqvist, F
Nordfors, L
Jansson, M
Thorne, A
Schalling, M
Arner, P
机构
[1] KAROLINSKA INST,HUDDINGE HOSP,DEPT MED,S-14186 STOCKHOLM,SWEDEN
[2] KAROLINSKA INST,HUDDINGE HOSP,RES CTR,S-14186 STOCKHOLM,SWEDEN
[3] KAROLINSKA INST,KAROLINSKA HOSP,DEPT MOL MED,NEUROGENET UNIT,S-14186 STOCKHOLM,SWEDEN
[4] KAROLINSKA INST,HUDDINGE HOSP,DEPT SURG,S-14186 STOCKHOLM,SWEDEN
关键词
ob gene; obesity; fat cells; body weight; messenger ribonucleic acid; OBESE GENE; MESSENGER-RNA;
D O I
10.1172/JCI119422
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The role of expression and secretion of the ob gene product, leptin, for the regulation of plasma leptin levels has been investigated in vitro using abdominal subcutaneous adipose tissue of 20 obese, otherwise healthy, and 11 nonobese women, Body mass index (BMI, mean +/- SEM; kg/m(2)) in the two groups was 41 +/- 2 and 23 +/- 1, respectively, Fat cell volume was 815 +/- 55 pl in the obese and 320 +/- 46 pl in the nonobese group, In the obese group, plasma leptin concentrations and adipose leptin mRNA (relative to gamma actin) were increased five and two times, respectively. Moreover, adipose tissue secretion rates per gram lipid weight or per fat cell number were also increased two and seven times, respectively, in the obese group, There were strong linear correlations (r = 0.6-0.8) between plasma leptin, leptin secretion, and leptin mRNA, All of these leptin measurements correlated strongly with BMI and fat cell volume (r = 0.7-0.9), About 60% of the variation in plasma leptin could be attributed to variations in leptin secretion rate, BMI, or fat cell volume. We conclude that elevated circulating levels of leptin in obese women above all result from accelerated secretion rates of the peptide from adipose tissue because of increased ob gene expression, However, leptin mRNA, leptin secretion, and circulating leptin levels are all more closely related to the stored amount of lipids in the fat cells of adipose tissue than they are to an arbitrary division into obese versus nonobese.
引用
收藏
页码:2398 / 2404
页数:7
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