Leginon: a system for fully automated acquisition of 1000 electron micrographs a day

被引：117

作者：

Potter, CS

Chu, H

Frey, B

Green, C

Kisseberth, N

Madden, TJ

Miller, KL

Nahrstedt, K

Pulokas, J

Reilein, A

Tcheng, D

Weber, D

Carragher, B

机构：

[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Comp Sci, Urbana, IL 61801 USA

[3] Natl Ctr Supercomp Applicat, Urbana, IL 61801 USA

来源：

ULTRAMICROSCOPY | 1999年 / 77卷 / 3-4期

基金：

美国国家科学基金会;

关键词：

microscopic methods; instrument control; automated data acquisition; three-dimensional electron microscopy;

D O I：

10.1016/S0304-3991(99)00043-1

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs, (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 161

页数：9

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CARRAGHER B, UNPUB MICROSC MICROA

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