Intracellular fate of retinyl acetate-loaded submicron delivery systems by in vitro intestinal epithelial cells: A comparison between whey protein-stabilised submicron droplets and micelles stabilised with polysorbate 80

被引：8

作者：

Benzaria, Amal ^{[1
]}

Chevalier-Lucia, Dominique ^{[1
]}

Picart-Palmade, Laetitia ^{[1
]}

Hue, Pauline ^{[1
]}

Lopez-Pedemonte, Tomas ^{[2
]}

Dumay, Eliane ^{[1
]}

机构：

[1] Univ Montpellier 2, UMR Ingn Agropolymeres & Technol Emergentes 1208, Equipe Biochim & Technol Alimentaires CC023, F-34095 Montpellier 5, France

[2] Univ Republica, Fac Quim, Dept Ciencia & Tecnol Alimentos, Montevideo, Uruguay

来源：

FOOD RESEARCH INTERNATIONAL | 2013年 / 51卷 / 02期

关键词：

Submicron emulsions; Ultra-high-pressure homogenisation; In vitro cell cultures; Whey proteins; Polysorbate; 80; Retinyl acetate; HIGH-PRESSURE HOMOGENIZATION; DYNAMIC HIGH-PRESSURE; RAW BOVINE-MILK; BETA-LACTOGLOBULIN; PHYSICOCHEMICAL CHARACTERISTICS; COMPETITIVE ADSORPTION; ANTIOXIDANT ACTIVITY; ALPHA-LACTALBUMIN; WATER EMULSIONS; CELLULAR UPTAKE;

D O I：

10.1016/j.foodres.2012.12.059

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Submicron peanut oil-in-water emulsion stabilised with whey proteins, was processed by ultra-high-pressure homogenisation at 200 MPa (first-stage, 2-pass homogenisation) and an initial emulsion temperature (T-in) of 24 degrees C. Retinyl acetate (RAC) was selected as a model of a lipophilic biomolecule to be carried by submicron droplets. For comparison, micelles of retinyl acetate (RAC-micelles) were prepared at atmospheric pressure using polysorbate 80 (Tween (R) 80) as emulsifier. Both types of bio-particles displayed submicron diameters. Their behaviour was investigated on TC7-cell monolayers to characterise their influence on monolayer integrity (TER measurements), cell metabolic activity (MTT-assay) and cell membrane integrity (LDH-leakage). TC7-cell exposure to submicron droplets UHPH-elaborated as shuttles for RAC, and stabilised with proteins, did not impair TC7-cell viability compared with control cells (without deposit) or cells exposed to RAC-micelles. Such results demonstrated UHPH-processing innocuity in terms of Novel,Food Regulation and implementation of UHPH as a novel technology. TC7-cell internalisation of whey proteins coating submicron oil droplets was observed using confocal microscopy and fluorescent probes. Cellular uptake of RAC was determined for both systems, after extraction from TC7 cells and HPLC quantitation. Results revealed RAC incorporation into TC7 cells and cellular turn-over of RAC into retinol for both systems without previous in vitro digestion. RAC bioaccessibility appeared yet better in the form of RAC-micelles than after entrapment into oily submicron droplets. Nevertheless, submicron droplets stabilised by proteins displayed a better physical stability than did RAC-micelles, which is the main feature for further developments of carrier systems. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：679 / 692

页数：14

共 81 条

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