Comparison of cytokine production in cultures of whole human blood and purified mononuclear cells

被引:122
作者
Yaqoob, P [1 ]
Newsholme, EA [1 ]
Calder, PC [1 ]
机构
[1] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
关键词
cytokine; mononuclear cells; whole blood;
D O I
10.1006/cyto.1998.0471
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to examine inter- and intra-individual variations in cytokine production, blood was collected from 48 healthy subjects on each of 4 occasions separated by 4 weeks. Whole blood (diluted 1:10) and mononuclear cell (MNC) cultures were stimulated for 24 h with either concanavalin A (Con A) or bacterial lipopolysaccharide (LPS) and the concentrations of IL-1 alpha, IL-1 beta, IL-2, TNF-alpha, IL-10 and IFN-gamma in the culture medium measured. There sere highly significant inter-individual variations in the production of each of the cytokines measured. However, the level of the production of each cytokine appeared to be characteristic of an individual. There mere significant correlations between production of each cytokine in whole blood and MNC cultures. It is concluded that there is significant inter-individual variation in cytokine production which is unaffected by time or by the stimulus used to elicit cytokine production, and that whole blood cultures can be used instead of MNC cultures to measure cytokine production. (C) 1999 Academic Press.
引用
收藏
页码:600 / 605
页数:6
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