Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion

被引：429

作者：

Martinez, Diego ^{[2
,3
]}

Challacombe, Jean ^{[2
]}

Morgenstern, Ingo

Hibbett, David ^{[4
]}

Schmoll, Monika ^{[5
]}

Kubicek, Christian P. ^{[5
]}

Ferreira, Patricia ^{[6
]}

Ruiz-Duenas, Francisco J. ^{[6
]}

Martinez, Angel T. ^{[6
]}

Kersten, Phil ^{[1
]}

Hammel, Kenneth E. ^{[1
]}

Wymelenberg, Amber Vanden ^{[7
]}

Gaskell, Jill ^{[1
]}

Lindquist, Erika

Sabat, Grzegorz ^{[9
]}

BonDurant, Sandra Splinter ^{[9
]}

Larrondo, Luis F. ^{[10
]}

Canessa, Paulo ^{[10
]}

Vicuna, Rafael ^{[10
]}

Yadav, Jagjit ^{[11
]}

Doddapaneni, Harshavardhan ^{[11
]}

Subramanian, Venkataramanan ^{[11
]}

Pisabarro, Antonio G. ^{[12
]}

Lavin, Jose L. ^{[12
]}

Oguiza, Jose A. ^{[12
]}

Master, Emma ^{[13
]}

Henrissat, Bernard ^{[14
,15
]}

Coutinho, Pedro M. ^{[14
,15
]}

Harris, Paul ^{[16
]}

Magnuson, Jon Karl ^{[17
]}

Baker, Scott E. ^{[17
]}

Bruno, Kenneth ^{[17
]}

Kenealy, William ^{[18
]}

Hoegger, Patrik J. ^{[19
]}

Kuees, Ursula ^{[19
]}

Ramaiya, Preethi ^{[16
]}

Lucash, Susan ^{[8
]}

Salamov, Asaf ^{[8
]}

Shapiro, Harris ^{[8
]}

Tu, Hank ^{[8
]}

Chee, Christine L.

Misra, Monica ^{[2
]}

Xie, Gary ^{[2
]}

Teter, Sarah ^{[16
]}

Yaver, Debbie ^{[16
]}

James, Tim ^{[20
]}

Mokrejs, Martin ^{[21
]}

Pospisek, Martin ^{[21
]}

Grigoriev, Igor V. ^{[8
]}

Brettin, Thomas ^{[2
]}

机构：

[1] Forest Prod Lab, Madison, WI 53726 USA

[2] Los Alamos Natl Lab, Joint Genome Inst, Los Alamos, NM 87545 USA

[3] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA

[4] Clark Univ, Dept Biol, Worcester, MA 01610 USA

[5] Vienna Univ Technol, Inst Chem Engn, Res Area Gene Technol & Appl Biochem, A-1060 Vienna, Austria

[6] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain

[7] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

[8] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA

[9] Univ Wisconsin, Ctr Biotechnol, Madison, WI 53706 USA

[10] Pontificia Univ Catolica Chile, Millennium Inst Fundamental & Appl Biol, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago 8331010, Chile

[11] Univ Cincinnati, Dept Environm Hlth, Cincinnati, OH 45267 USA

[12] Univ Publ Navarra, Genet & Microbiol Res Grp, Pamplona 31006, Spain

[13] Univ Toronto, Toronto, ON M5S 3E5, Canada

[14] Univ Aix Marseille 1, CNRS, UMR 6098, F-13288 Marseille, France

[15] Univ Aix Marseille 2, CNRS, UMR 6098, F-13288 Marseille, France

[16] Novozymes Inc, Davis, CA 95618 USA

[17] Pacific NW Natl Lab, Richland, WA 99352 USA

[18] Mascoma Inc, Lebanon, NH 03766 USA

[19] Univ Gottingen, Busgen Inst, D-37077 Gottingen, Germany

[20] Univ Michigan, Dept Ecol & Evolut Biol, Ann Arbor, MI 48109 USA

[21] Charles Univ Prague, Fac Sci, CR-12844 Prague, Czech Republic

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 06期

基金：

美国国家卫生研究院;

关键词：

cellulose; fenton; lignin; cellulase; brown-rot; PHANEROCHAETE-CHRYSOSPORIUM; ALCOHOL OXIDASE; OXALIC-ACID; WHITE-ROT; CELLULOSE; DEGRADATION; SEQUENCE; LIGNIN; CLASSIFICATION; IDENTIFICATION;

D O I：

10.1073/pnas.0809575106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exo-cellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H2O2. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe( II) and H2O2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.

引用

页码：1954 / 1959

页数：6

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