Effect of Surface Topography of Collagen Scaffolds on Cytotoxicity and Osteoblast Differentiation

被引：23

作者：

Cortizo, Ana M. ^{[1
]}

Ruderman, Graciela ^{[2
]}

Correa, Gimena ^{[1
]}

Mogilner, Ines G. ^{[2
]}

Tolosa, Eduardo J. ^{[2
]}

机构：

[1] Univ Nacl La Plata, Fac Ciencias Exactas, GIOMM, Dept Ciencias Biol, RA-1900 La Plata, Buenos Aires, Argentina

[2] Univ Nacl La Plata, Fac Ciencias Exactas, IFLYSIB CONICET UNLP CIC, RA-1900 La Plata, Buenos Aires, Argentina

来源：

JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2012年 / 2卷 / 02期

关键词：

Collagen; Bone Tissue Engineering; Adhesion; Proliferalion; Alkaline Phosphatase; Mineralization; Cytotoxocity; BONE; CELLS; ADHESION; BIOCOMPATIBILITY; BIOMATERIALS; MODEL;

D O I：

10.1166/jbt.2012.1039

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

We have developed a biomimetic scaffold for tissue engineering using bovine collagen with different topographic characteristics, using matrices with random or parallel-arranged collagen fibres. Scaffolds were characterized by SEM, water contact angle and mechanical test. Biocompatiblitiy studies were performed with MC3T3E1 pre-osteoblasts, and adhesion, proliferation, differentiation and mineralization were assessed. Random film showed a smooth surface, while the ordered membrane revealed a grooved structure with channels separated 265 mu m from each other. Water contact angle was dependent on the direction of observation and the mechanical evaluation showed a lower resistance to traction and a greater ductility for the ordered membrane. Adhesion, proliferation, alkaline phosphatase activity and mineralization were significantly improved when cells were grown on the ordered-collagen matrix. There was no significant increase in pro-inflammatory cytokine release from cells grown on both random and ordered-collagen films. This material could be useful for bone tissue regeneration.

引用

页码：125 / 132

页数：8

共 27 条

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