S100B secretion is stimulated by IL-1β in glial cultures and hippocampal slices of rats: Likely involvement of MAPK pathway

被引：54

作者：

de Souza, Daniela F. ^{[1
]}

Leite, Marina C. ^{[1
]}

Quincozes-Santos, Andre ^{[1
]}

Nardin, Patricia ^{[1
]}

Tortorelli, Lucas S. ^{[1
]}

Rigo, Mauricio M. ^{[1
]}

Gottfried, Carmem ^{[1
]}

Leal, Rodrigo B. ^{[2
]}

Goncalves, Carlos-Alberto ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICBS, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Bioquim, Florianopolis, SC, Brazil

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2009年 / 206卷 / 1-2期

关键词：

Astrocyte; Interleukin-1; beta; MAPK; NF-kappa B; S100B secretion; ALZHEIMERS-DISEASE; METHODOLOGICAL FEATURES; CORTICAL ASTROCYTES; NERVOUS-SYSTEM; BETA; PROTEIN; BRAIN; NEURODEGENERATION; CELLS; INTERLEUKIN-1;

D O I：

10.1016/j.jneuroim.2008.10.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

S100B is an astrocyte-derived cytokine implicated in the IL-1 beta-triggered cytokine cycle in Alzheimer's disease. However, the secretion of S100B following stimulation by IL-1 beta has not been directly demonstrated. We investigated S100B secretion in cortical primary astrocyte cultures, C6 glioma cells and acute hippocampal slices exposed to IL-1 beta. S100B secretion was induced by IL-1 beta in all preparations, involving MAPK pathway and, apparently, NF-kappa B signaling. Astrocytes and C6 cells exhibited different sensitivities to IL-1 beta. These results suggest that IL-1 beta-induced S100B secretion is a component of the neuroinflammatory response, which would support the involvement of S100B in the genesis of neurodegenerative diseases. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：52 / 57

页数：6

共 47 条

[1] Inflammation in central nervous system injury [J].

Allan, SM ;

Rothwell, NJ .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2003, 358 (1438) :1669-1677

[2] Interleukin-1 and neuronal injury [J].

Allan, SM ;

Tyrrell, PJ ;

Rothwell, NJ .

NATURE REVIEWS IMMUNOLOGY, 2005, 5 (08) :629-640

[3] Loss of IκB-β is associated with prolonged NF-κB activity in human glial cells [J].

Bourke, E ;

Kennedy, EJ ;

Moynagh, PN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (51) :39996-40002

[4] Transcriptional regulation of the human S100 beta gene [J].

Castets, F ;

Griffin, WST ;

Marks, A ;

VanEldik, LJ .

MOLECULAR BRAIN RESEARCH, 1997, 46 (1-2) :208-216

[5] Protein kinase C-βII is an apoptotic lamin kinase in polyomavirus-transformed, etoposide-treated pyF111 rat fibroblasts [J].

Chiarini, A ;

Whitfield, JF ;

Armato, U ;

Dal Pra, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (21) :18827-18839

[6] Intracellular Ca2+ and Zn2+ levels regulate the alternative cell density-dependent secretion of S100B in human glioblastoma cells [J].

Davey, GE ;

Murmann, P ;

Heizmann, CW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (33) :30819-30826

[7] S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles [J].

Donato, R .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2001, 33 (07) :637-668

[8] The significance of neuroinflammation in understanding Alzheimer's disease [J].

Eikelenboom, P. ;

Veerhuis, R. ;

Scheper, W. ;

Rozemuller, A. J. M. ;

van Gool, W. A. ;

Hoozemans, J. J. M. .

JOURNAL OF NEURAL TRANSMISSION, 2006, 113 (11) :1685-1695

[9]

FENA LA, 1995, BRAIN RES MOL BRAIN, V34, P118

[10] MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes [J].

Fernandes, Adelaide ;

Falcao, Ana S. ;

Silva, Rui F. M. ;

Brito, Maria A. ;

Brites, Dora .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2007, 25 (04) :1058-1068

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