A Baculovirus anti-apoptosis gene homolog of the Trichoplusia ni granulovirus

被引:7
作者
Bideshi, DK
Anwar, AT
Federici, BA [1 ]
机构
[1] Univ Calif Riverside, Grad Program Genet, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Grad Program Microbiol, Riverside, CA 92521 USA
[3] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA
关键词
baculovirus; granulovirus; Trichoplusia ni; inhibitor of apoptosis; IAP; baculovirus imperfect repeated sequence motif; RING finger;
D O I
10.1023/A:1008148922330
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
An inhibitor of apoptosis (iap) gene homolog (Tn-iap) of the Trichoplusia ni granulovirus (TnGV) was cloned, sequenced and mapped on the genome of TnGV. Tn-iap encoded a protein (Tn-IAP) of 301 amino acids with a predicted molecular mass of 35 kDa. The Tn-IAP contained the two sequence motifs, BIRs and RING finger, characteristic of IAP proteins, and shared identities of 21-27% and similarities of 28-53% with IAP proteins of Cydia pomonella GV (Cp-IAP), Orgyia pseudotsugata multinucleocapsid nucleopolyhedrovirus (MNPV) (Op-IAP1, 3), Autographa californica MNPV (Ac-IAP1), Bombyx mori NPV (Bm-IAP1), Lymantria dispar MNPV (Ld-IAP3) and Buzura suppressaria single nucleocapsid NPV (Bs-IAP1). However, Tn-IAP shared no significant homology with baculovirus IAP2 proteins. Using an antisense Tn-iap probe, two major transcripts of approximately 800 nt and 1600 nt were detected by Northern blot analysis of RNA extracted from the fat body of T. ni larvae infected with the TnGV. Unlike Cp-IAP and Op-IAP3, however, Tn-IAP did not rescue virion occlusion in SF21 cells infected with a p35-deficient AcMNPV mutant. Tn-IAP's synthesis in vivo but failure to rescue p35-deficient AcMNPV in SF21 cells suggests it is a functional IAP that is only effective in certain cell types.
引用
收藏
页码:95 / 101
页数:7
相关论文
共 32 条
[1]   The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome [J].
Ahrens, CH ;
Russell, RLQ ;
Funk, CJ ;
Evans, JT ;
Harwood, SH ;
Rohrmann, GF .
VIROLOGY, 1997, 229 (02) :381-399
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   THE COMPLETE DNA-SEQUENCE OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].
AYRES, MD ;
HOWARD, SC ;
KUZIO, J ;
LOPEZFERBER, M ;
POSSEE, RD .
VIROLOGY, 1994, 202 (02) :586-605
[4]   Molecular characterization and expression of the Trichoplusia ni granulovirus helicase gene [J].
Bideshi, DK ;
Hice, RH ;
Ge, B ;
Federici, BA .
JOURNAL OF GENERAL VIROLOGY, 1998, 79 :1309-1319
[5]   AN APOPTOSIS-INHIBITING GENE FROM A NUCLEAR POLYHEDROSIS-VIRUS ENCODING A POLYPEPTIDE WITH CYS/HIS SEQUENCE MOTIF [J].
BIRNBAUM, MJ ;
CLEM, RJ ;
MILLER, LK .
JOURNAL OF VIROLOGY, 1994, 68 (04) :2521-2528
[6]   PREVENTION OF APOPTOSIS BY A BACULOVIRUS GENE DURING INFECTION OF INSECT CELLS [J].
CLEM, RJ ;
FECHHEIMER, M ;
MILLER, LK .
SCIENCE, 1991, 254 (5036) :1388-1390
[7]   APOPTOSIS REDUCES BOTH THE INVITRO REPLICATION AND THE INVIVO INFECTIVITY OF A BACULOVIRUS [J].
CLEM, RJ ;
MILLER, LK .
JOURNAL OF VIROLOGY, 1993, 67 (07) :3730-3738
[8]   CONTROL OF PROGRAMMED CELL-DEATH BY THE BACULOVIRUS GENES P35 AND IAP [J].
CLEM, RJ ;
MILLER, LK .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (08) :5212-5222
[9]  
Clem RJ, 1996, CELL DEATH DIFFER, V3, P9
[10]   AN APOPTOSIS-INHIBITING BACULOVIRUS GENE WITH A ZINC FINGER-LIKE MOTIF [J].
CROOK, NE ;
CLEM, RJ ;
MILLER, LK .
JOURNAL OF VIROLOGY, 1993, 67 (04) :2168-2174