Agrobacterium tumefaciens-mediated transformation of Mentha spicata and Mentha arvensis

被引：14

作者：

Diemer, F ^{[1
]}

Caissard, JC ^{[1
]}

Moja, S ^{[1
]}

Jullien, F ^{[1
]}

机构：

[1] Univ St Etienne, Fac Sci & Tech, Lab Biotechnol Vegetales Appl Plantes Aromat & Me, F-42023 St Etienne 2, France

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1999年 / 57卷 / 01期

关键词：

Agrobacterium tumefaciens; genetic transformation; GUS; Mentha spicata; Mentha arvensis;

D O I：

10.1023/A:1006280521212

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The stable integration of GUS and NPTII genes in Mentha arvensis and M. spicata has been achieved by Agrobacterium tumefaciens-mediated gene transfer. Transformation assays were performed by cocultivating plant leaf disks with either GV2260/GI or EHA105/MOG Agrobacterium strains. Transgenic plants were selected on medium containing 150 mg l(-1) kanamycin. Transgene presence and structure was studied by the use of PCR analysis and Southern blot hybridization. Transgene expression was evaluated by RT-PCR and transgene product activity by a histoenzymatic GUS assay.

引用

页码：75 / 78

页数：4

共 9 条

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