Large-scale production of VHH antibody fragments by Saccharomyces cerevisiae

被引：41

作者：

Thomassen, YE

Meijer, W

Sierkstra, L

Verrips, CT

机构：

[1] Univ Utrecht, Dept Mol Cell Biol, NL-3584 CH Utrecht, Netherlands

[2] Univ Utrecht, Inst Biomembranes, NL-3584 CH Utrecht, Netherlands

[3] Unilever Res Labs Vlaardingen, NL-3133 AT Vlaardingen, Netherlands

[4] Biotechnol Applicat Ctr, Naarden, Netherlands

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 03期

关键词：

antibody; V-HH; bulk production; secretion; S; cerevisiae;

D O I：

10.1016/S0141-0229(01)00497-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the large-scale production of V-HH antibody fragments, raised against the azo-dye RR-6, by the yeast Saccharomyces cerevisiae. The secretion efficiency of the V-HH fragments was correlated with their overall hydrophobicity. With a 15 m(3) fed-batch fermentation, 1.3 kg V(HH)s were produced. Purification was done by removal of biomass and product concentration. During induction, the specific production rate q(p) was 0.213 g.[kg dryweight](-1).h(-1). (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：273 / 278

页数：6

共 32 条

[1]

ALBERGHINA L, 1991, BIOTECHNOL APPL BIOC, V14, P82

[2]

ASHLEY NV, 1990, J CHEM TECHNOL BIOT, V49, P381

[3] AFFINITY PANNING OF A LIBRARY OF PEPTIDES DISPLAYED ON BACTERIOPHAGES REVEALS THE BINDING-SPECIFICITY OF BIP [J].

BLONDELGUINDI, S ;

CWIRLA, SE ;

DOWER, WJ ;

LIPSHUTZ, RJ ;

SPRANG, SR ;

SAMBROOK, JF ;

GETHING, MJH .

CELL, 1993, 75 (04) :717-728

[4]

DEHAARD JJW, UNPUB INHIBITION PHA

[5]

*EMBL, AJ236103R5 EMBL

[6]

*EMBL, AJ236107R9 EMBL

[7]

*EMBL, AJ236105R7 EMBL

[8]

*EMBL, AJ236100R2 EMBL

[9] Recent advances in the large-scale production of antibody fragments using lower eukaryotic microorganisms [J].

Frenken, LGJ ;

Hessing, JGM ;

Van den Hondel, CAMJJ ;

Verrips, CT .

RESEARCH IN IMMUNOLOGY, 1998, 149 (06) :589-599

[10] Isolation of antigen specific Llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae [J].

Frenken, LGJ ;

van der Linden, RHJ ;

Hermans, PWJJ ;

Bos, JW ;

Ruuls, RC ;

de Geus, B ;

Verrips, CT .

JOURNAL OF BIOTECHNOLOGY, 2000, 78 (01) :11-21

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