A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

被引：9

作者：

McKinnon, W

Lord, GA

Forni, LG

Peron, JMR

Hilton, PJ

机构：

[1] St Thomas Hosp, Renal Res Lab, Dept Med, London SE1 7EH, England

[2] Univ London Birkbeck Coll, Sch Biol & Chem Sci, MRC, Bioanalyt Sci Grp, London WC1E 7HX, England

[3] Worthing Dist Hosp, Dept Crit Care Med, Worthing BN11 2DH, W Sussex, England

[4] Brighton & Sussex Med Sch, Brighton, E Sussex, England

[5] Kingston Univ, Sch Pharm & Chem, Kingston upon Thames KT1 2EE, Surrey, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 833卷 / 02期

关键词：

acidosis; patients; plasma; ultrafiltrate; krebs cycle; anion gap; intermediatry metabolism; liquid chromatography; mass spectrometry;

D O I：

10.1016/j.jchromb.2006.01.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：179 / 185

页数：7

共 11 条

[1] RAPID PROFILING OF PLASMA ORGANIC-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].