Crystallization of a precursor penicillin acylase from Escherichia coli

被引:5
作者
Hewitt, L [1 ]
Kasche, V
Lummer, K
Rieks, A
Wilson, KS
机构
[1] Univ York, Dept Chem, York YO1 5DD, N Yorkshire, England
[2] Tech Univ Hamburg Harburg, AB Biotechnol 2, D-21071 Hamburg, Germany
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词
D O I
10.1107/S090744499900195X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The crystallization of a mutant precursor penicillin acylase [penicillin amidohydrolase (amidase) E.C 3.5.1.11] from Escherichia coli W (ATCC 11105) using the hanging-drop method is reported. The crystals are in space group P1 with unit-cell parameters a = 51.04, b = 63.58, c = 71.17 Angstrom, alpha = 103.0, beta = 110.6, gamma = 105.3 degrees, with one molecule in the asymmetric unit, and diffract to 1.8 Angstrom using synchrotron radiation.
引用
收藏
页码:1052 / 1054
页数:3
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