Hoxa2 knockdown in Xenopus results in hyoid to mandibular homeosis

被引:46
作者
Baltzinger, M
Ori, M
Pasqualetti, M
Nardi, I
Rijli, FM
机构
[1] ULP, INSERM, CNRS,Inst Genet & Biol Mol & Cellulaire, UMR 7104,CU Strasbourg, F-67404 Illkirch Graffenstaden, France
[2] CNRS, UMR 7175, ESBS, F-67412 Illkirch Graffenstaden, France
[3] Univ Pisa, Lab Biol Cellulare & Sviluppo, Dipartimento Fisiol & Biochim, I-56100 Pisa, Italy
关键词
Hox; homeotic transformation; morpholino; neural crest; cartilage; jaw; evolution;
D O I
10.1002/dvdy.20567
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The skeletal structures of the face and throat are derived from cranial neural crest cells (NCCs) that migrate from the embryonic neural tube into a series of branchial arches (BAs). The first arch (BA1) gives rise to the upper and lower jaw cartilages, whereas hyoid structures are generated from the second arch (BA2). The Hox paralogue group 2 (PG2) genes, Hoxa2 and Hoxb2, show distinct roles for hyoid patterning in tetrapods and fishes. In the mouse, Hoxa2 acts as a selector of hyoid identity, while its paralogue Hoxb2 is not required. On the contrary, in zebrafish Hoxa2 and Hoxb2 are functionally redundant for hyoid arch patterning. Here, we show that in Xenopus embryos morpholino-induced functional knockdown of Hoxa2 is sufficient to induce homeotic changes of the second arch cartilage. Moreover, Hoxb2 is downregulated in the BA2 of Xenopus embryos, even though initially expressed in second arch NCCs, similar to mouse and unlike in zebrafish. Finally, Xbap, a gene involved in jaw joint formation, is selectively upregulated in the BA2 of Hoxa2 knocked-down frog embryos, supporting a hyoid to mandibular change of NCC identity. Thus, in Xenopus Hoxa2 does not act redundantly with Hoxb2 for BA2 patterning, similar to mouse and unlike in fish. These data bring novel insights into the regulation of Hox PG2 genes and hyoid patterning in vertebrate evolution and suggest that Hoxa2 function is required at late stages of BA2 development.
引用
收藏
页码:858 / 867
页数:10
相关论文
共 55 条
  • [1] [Anonymous], [No title captured]
  • [2] Baltzinger M, 1999, DEV DYNAM, V216, P420, DOI 10.1002/(SICI)1097-0177(199912)216:4/5<420::AID-DVDY10>3.0.CO
  • [3] 2-C
  • [4] Barrow JR, 2000, DEVELOPMENT, V127, P933
  • [5] Barrow JR, 1996, DEVELOPMENT, V122, P3817
  • [6] Developmental origins and evolution of jaws: new interpretation of "maxillary" and "mandibular"
    Cerny, R
    Lwigale, P
    Ericsson, R
    Meulemans, D
    Epperlein, HH
    Bronner-Fraser, M
    [J]. DEVELOPMENTAL BIOLOGY, 2004, 276 (01) : 225 - 236
  • [7] Couly G, 1998, DEVELOPMENT, V125, P3445
  • [8] COULY GF, 1993, DEVELOPMENT, V117, P409
  • [9] Creuzet S, 2002, DEVELOPMENT, V129, P4301
  • [10] Hoxa2 and Hoxb2 control dorsoventral patterns of neuronal development in the rostral hindbrain
    Davenne, M
    Maconochie, MK
    Neun, R
    Pattyn, A
    Chambon, P
    Krumlauf, R
    Rijli, FM
    [J]. NEURON, 1999, 22 (04) : 677 - 691