Heparan sulfate C5-epimerase is essential for heparin biosynthesis in mast cells

被引：43

作者：

Feyerabend, TB

Li, JP

Lindahl, U

Rodewald, HR

机构：

[1] Univ Uppsala, Dept Med Biochem & Microbiol, Biomed Ctr, SE-75123 Uppsala, Sweden

[2] Univ Ulm, Dept Immunol, D-89070 Ulm, Germany

来源：

NATURE CHEMICAL BIOLOGY | 2006年 / 2卷 / 04期

关键词：

D O I：

10.1038/nchembio777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biosynthesis of heparin, a mast cell-derived glycosaminoglycan with widespread importance in medicine, has not been fully elucidated. In biosynthesis of heparan sulfate (HS), a structurally related polysaccharide, HS glucuronyl C5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) residues. We have generated Hsepi-null mouse mutant mast cells, and we show that the same enzyme catalyzes the generation of IdoA in heparin and that 'heparin' lacking IdoA shows a distorted O-sulfation pattern.

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页码：195 / 196

页数：2

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