High-resolution ChIP-chip analysis reveals that the Drosophila MSL complex selectively identifies active genes on the male X chromosome

被引:172
作者
Alekseyenko, AA
Larschan, E
Lai, WR
Park, PJ [1 ]
Kuroda, MI
机构
[1] Brigham & Womens Hosp, Howard Hughes Med Inst, 75 Francis St, Boston, MA 02115 USA
[2] Harvard Univ, Brigham & Womens Hosp, Partners Ctr Genet & Genom, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[4] Childrens Hosp, Informat Program, Boston, MA 02115 USA
关键词
ChIP-chip; MSL complex; gene expression; chromatin modification; histone modification; dosage compensation;
D O I
10.1101/gad.1400206
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
X-chromosome dosage compensation in Drosophila requires the male-specific lethal (MSL) complex, which up-regulates gene expression from the single male X chromosome. Here, we define X-chromosome-specific MSL binding at high resolution in two male cell lines and in late-stage embryos. We find that the MSL complex is highly enriched over most expressed genes, with binding biased toward the 3 ' end of transcription units. The binding patterns are largely similar in the distinct cell types, with similar to 600 genes clearly bound in all three cases. Genes identified as clearly bound in one cell type and not in another indicate that attraction of MSL complex correlates with expression state. Thus, sequence alone is not sufficient to explain MSL targeting. We propose that the MSL complex recognizes most X-linked genes, but only in the context of chromatin factors or modifications indicative of active transcription. Distinguishing expressed genes from the bulk of the genome is likely to be an important function common to many chromatin organizing and modifying activities.
引用
收藏
页码:848 / 857
页数:10
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