Perspectives of immobilized-metal affinity chromatography

被引：431

作者：

Gaberc-Porekar, V

Menart, V

机构：

[1] Natl Inst Chem, SI-1000 Ljubljana, Slovenia

[2] Lek DD, R&D, Dept Biotechnol, SI-1000 Ljubljana, Slovenia

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

IMAC; immobilized-metal affinity chromatography; recombinant proteins; histidine tags; pharmaceutical proteins;

D O I：

10.1016/S0165-022X(01)00207-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized Metal-Affinity Chromatography (IMAC) represents a relatively new separation technique that is primarily appropriate for the purification of proteins with natural surface-exposed histidine residues and for recombinant proteins with engineered histidine tags or histidine clusters. Because the method has gained broad popularity in recent years, the main recent developments in the field of new sorbents, techniques and possible applications are discussed in this article. Advantages of the method and new prospects are described as well as the problems and concerns that appear when the method is to be used for production of pharmaceutical-grade proteins. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：335 / 360

页数：26

共 128 条

[1] Preparation of magnetic immobilized metal affinity separation media and its use in the isolation of proteins [J].