Automated screening for mutants affecting dopaminergic-neuron specification in C-elegans

被引：63

作者：

Doitsidou, Maria ^{[1
]}

Flames, Nuria ^{[1
]}

Lee, Albert C. ^{[1
]}

Boyanov, Alexander ^{[1
]}

Hobert, Oliver ^{[1
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, Med Ctr, New York, NY 10032 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/NMETH.1250

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately.

引用

页码：869 / 872

页数：4

共 15 条

[1] Midbrain dopamine neuron differentiation: Factors and fates
Abeliovich, Asa
Hammond, Rachel
[J]. DEVELOPMENTAL BIOLOGY, 2007, 304 (02) : 447 - 454
[2] Mos1-mediated insertional mutagenesis in Caenorhabditis elegans
Boulin, Thomas
Bessereau, Jean-Louis
[J]. NATURE PROTOCOLS, 2007, 2 (05) : 1276 - 1287
[3] BRENNER S, 1974, GENETICS, V77, P71
[4] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION
CHALFIE, M
TU, Y
EUSKIRCHEN, G
WARD, WW
PRASHER, DC
[J]. SCIENCE, 1994, 263 (5148) : 802 - 805
[5] Mechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans
Chase, DL
Pepper, JS
Koelle, MR
[J]. NATURE NEUROSCIENCE, 2004, 7 (10) : 1096 - 1103
[6] Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans
Chung, Kwanghun
Crane, Matthew M.
Lu, Hang
[J]. NATURE METHODS, 2008, 5 (07) : 637 - 643
[7] Rapid single nucleotide polymorphism mapping in C-elegans -: art. no. 118
Davis, MW
Hammarlund, M
Harrach, T
Hullett, P
Olsen, S
Jorgensen, EM
[J]. BMC GENOMICS, 2005, 6 (1)
[8] Guenther C, 1996, DEVELOPMENT, V122, P3509
[9] Neuronal fate determinants of adult olfactory bulb neurogenesis
Hack, MA
Saghatelyan, A
de Chevigny, A
Pfeifer, A
Ashery-Padan, R
Lledo, PM
Götz, M
[J]. NATURE NEUROSCIENCE, 2005, 8 (07) : 865 - 872
[10] The art and design of genetic screens:: Caenorhabditis elegans
Jorgensen, EM
Mango, SE
[J]. NATURE REVIEWS GENETICS, 2002, 3 (05) : 356 - 369

← 1 2 →