Stir bar sorptive extraction-liquid desorption applied to the analysis of hop-derived bitter acids in beer by micellar electrokinetic chromatography

被引：15

作者：

De Villiers, A

Vanhoenacker, G

Lynen, F

Sandra, P

机构：

[1] Res Inst Chromatog, B-8500 Kortrijk, Belgium

[2] Univ Stellenbosch, CENSSUS, Dept Chem, Matieland, South Africa

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 4-5期

关键词：

beer; hop bitter acids; liquid desorption; micellar electrokinetic chromatography; stir bar sorptive extraction;

D O I：

10.1002/elps.200305758

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stir bar sorptive extraction-liquid desorption (SBSE-LD) has been applied as an efficient sample preparation method for the analysis of beer bitter acids. Extracts free of almost all interfering compounds were obtained, allowing simultaneous analysis of iso-alpha-acids and reduced iso-alpha-acids. A robust micellar electrokinetic chromatography (MEKC) method was developed that enables fast separation of iso-alpha-acids and reduced iso-alpha-acids. Quantitative data are in good agreement with results obtained by high-performance liquid chromatography (HPLC) using direct beer injection.

引用

页码：664 / 669

页数：6

共 33 条

[1]

Baltussen E, 1999, J MICROCOLUMN SEP, V11, P737, DOI 10.1002/(SICI)1520-667X(1999)11:10<737::AID-MCS7>3.0.CO

[2]

2-4

[3]

Clark JG, 1998, J AM SOC BREW CHEM, V56, P76

[4]

David F, 2003, LC GC N AM, V21, P108

[5] THE HISTORY AND ANALYTICAL-CHEMISTRY OF BEER BITTER ACIDS [J].

DEKEUKELEIRE, D ;

VINDEVOGEL, J ;

SZUCS, R ;

SANDRA, P .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1992, 11 (08) :275-280

[6]

HARMS D, 2001, GERSTEL APPL NOTE 5

[7] DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SYSTEM FOR THE ANALYSIS OF HOP BITTER ACIDS [J].

HERMANSLOKKERBOL, ACJ ;

VERPOORTE, R .

JOURNAL OF CHROMATOGRAPHY A, 1994, 669 (1-2) :65-73

[8]

Hofte AJP, 1998, J AM SOC BREW CHEM, V56, P118

[9]

Kuroiwa Y., 1963, P AM SOC BREW CHEM, V28, P181

[10]

MEYLAN WM, SOFTWARE KOWWIN VERS

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