Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for proteomic studies

被引：15

作者：

Abdalla, Kamal Omer ^{[1
]}

Thomson, Jennifer Ann ^{[1
]}

Rafudeen, Muhammad Suhail ^{[1
]}

机构：

[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 384卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1016/j.ab.2008.09.049

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and Subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：365 / 367

页数：3

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