Evaluation of two avian influenza type A rapid antigen tests under Indonesian field conditions

被引：12

作者：

Loth, Leo ^{[1
]}

Prijono, Walujo Budi ^{[2
]}

Wibawa, Hendra ^{[2
]}

Usman, Tri Bhakti ^{[2
]}

机构：

[1] Food & Agr Org United Nations, Jakarta 10250, Indonesia

[2] Dis Invest Ctr Reg IV, Wates, Yogyakarta, Indonesia

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2008年 / 20卷 / 05期

关键词：

Antigen test; avian influenza; H5N1; poultry; sensitivity; specificity;

D O I：

10.1177/104063870802000519

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The current study evaluated the test characteristics of 2 commercially available rapid antigen tests for highly pathogenic avian influenza. Diagnostic specimens were collected from free-ranging village chickens in Indonesia. A total of 174 healthy, sick, and dead birds were included in the study. The relative diagnostic test sensitivity and the relative diagnostic test specificity were calculated by using real-time reverse transcription polymerase chain reaction (RT-PCR) as the confirmatory diagnosis. The AnigenR Rapid AIV Ag test had a relative diagnostic sensitivity of 0.69 (95% confidence interval [CI]: 0.56-0.80) and a relative diagnostic specificity of 0.98 (95% CI: 0.93-0.99). The Flu Detect (TM) Antigen Capture test strip had a relative diagnostic sensitivity of 0.71 (95% CI: 0.58-0.82) and a relative diagnostic specificity of 0.98 (95% CI: 0.930.99). These tests are a valuable tool for the Indonesian avian influenza control program by reliably and quickly detecting Influenza A virus from oropharyngeal swabs from sick or dying chickens.

引用

页码：642 / 644

页数：3

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