A structural analysis of cytoskeleton components during the execution phase of apoptosis

被引:14
作者
Atencia, R [1 ]
GarciaSanz, M [1 ]
PerezYarza, G [1 ]
Asumendi, A [1 ]
Hilario, E [1 ]
Arechaga, J [1 ]
机构
[1] UNIV BASQUE COUNTRY, FAC MED, DEPT BIOL CELULAR & CIENCIAS MORFOL, E-48940 VIZCAYA, SPAIN
关键词
actin; actin-binding proteins; apoptosis; F9 embryonal carcinoma cells;
D O I
10.1007/BF01287565
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
During the execution phase of apoptosis, the cell undergoes a set of morphological changes which reveal the activation of a complex machinery leading the cell to its disruption into small, spherical, membrane-bounded fragments called apoptotic bodies. In the present study, we have focused on the implications of the microfilament network in the early stages of the active phase of apoptosis. By using confocal microscopy, we have analysed the location of the actin microfilaments and two actin-binding proteins, alpha-actinin and myosin, in F9 embryonal carcinoma cells undergoing apoptosis during the stages previous to their fragmentation. Our results show that these proteins locate in the centre of the disrupting cell and form a three-dimensional structure which suggests the existence of a fully functional contractile system involved in the fragmentation of the cell and the formation of apoptotic bodies.
引用
收藏
页码:163 / 169
页数:7
相关论文
共 24 条
[1]  
ARENDS MJ, 1991, INT REV EXP PATHOL, V32, P223
[2]   APOPTOSIS DURING RETINOIC ACID-INDUCED DIFFERENTIATION OF F9 EMBRYONAL CARCINOMA-CELLS [J].
ATENCIA, R ;
GARCIASANZ, M ;
UNDA, F ;
ARECHAGA, J .
EXPERIMENTAL CELL RESEARCH, 1994, 214 (02) :663-667
[3]   MICROFILAMENT REORGANIZATION DURING APOPTOSIS - THE ROLE OF GAS2, A POSSIBLE SUBSTRATE FOR ICE-LIKE PROTEASES [J].
BRANCOLINI, C ;
BENEDETTI, M ;
SCHNEIDER, C .
EMBO JOURNAL, 1995, 14 (21) :5179-5190
[4]   EFFECTS OF CYTOCHALASIN AND PHALLOIDIN ON ACTIN [J].
COOPER, JA .
JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1473-1478
[5]  
COTTER TG, 1992, CANCER RES, V52, P997
[6]   APOPTOSIS - LESSONS FROM IN-VITRO SYSTEMS [J].
EARNSHAW, WC .
TRENDS IN CELL BIOLOGY, 1995, 5 (06) :217-220
[7]  
FESUS L, 1991, EUR J CELL BIOL, V56, P170
[8]  
FOX JEB, 1987, BLOOD, V69, P537
[9]   THE MEMBRANE SKELETON - A DISTINCT STRUCTURE THAT REGULATES THE FUNCTION OF CELLS [J].
FOX, JEB ;
BOYLES, JK .
BIOESSAYS, 1988, 8 (01) :14-18
[10]  
GARCIASANZ M, 1996, INT J DEV BIOL S1, V40, P195