Barley viability during storage: Use of magnetic resonance as a potential tool to study viability loss

被引:27
作者
Gruwel, MLH
Yin, XS
Edney, MJ
Schroeder, SW
MacGregor, AW
Abrams, S
机构
[1] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
[2] Prairie Malt Ltd, Cargill Malt Amer, Biggar, SK S0K 0M0, Canada
[3] Canadian Grain Commiss, Grain Res Lab, Winnipeg, MB R3C 3G8, Canada
关键词
H-1; NMR; imbibition; relaxation time; MR imaging; germination; alpha-amylase;
D O I
10.1021/jf0108617
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Malting-quality barley samples of the varieties Harrington, Manley, and TR118, each from two locations in Saskatchewan, were collected directly from the producers and sent to China for storage. At regular intervals samples were shipped back to Canada for analysis consisting of germination studies, alpha-amylase tests, magnetic resonance imaging (MRI), and magnetic relaxation (NMR) studies. Samples showing a decrease in germinative energy and elevated levels of alpha-amylase also showed a rapid uptake of water in the area between the embryo and the endosperm as observed by MRI. Using NMR relaxation experiments, viable and nonviable barley samples could be distinguished after 2 h of imbibition.
引用
收藏
页码:667 / 676
页数:10
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