Effects of Lycopene on Protein Expression in Human Primary Prostatic Epithelial Cells

被引:31
作者
Qiu, Xi [1 ]
Yuan, Yang [1 ]
Vaishnav, Avani [2 ]
Tessel, Michael A. [2 ]
Nonn, Larisa [2 ]
van Breemen, Richard B. [1 ]
机构
[1] Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA
[2] Univ Illinois, Coll Pharm, Dept Pathol, Chicago, IL 60612 USA
关键词
OXIDATIVE DNA-DAMAGE; LIPID-PEROXIDATION; CANCER PATIENTS; APOPTOSIS; MODELS; GROWTH; SUPPLEMENTATION; PROGRESSION; HYPERPLASIA; INHIBITION;
D O I
10.1158/1940-6207.CAPR-12-0364
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Clinical trials and animal studies have suggested that lycopene, the red carotenoid found in tomatoes, might be useful for the prevention of prostate cancer in the diet or as a dietary supplement through a variety of chemoprevention mechanisms. As most mechanism of action studies have used prostate cancer cells or males with existing prostate cancer, we investigated the effects of lycopene on protein expression in human primary prostatic epithelial cells. After treatment with lycopene at a physiologically relevant concentration (2 mu mol/L) or placebo for 48 hours, the primary prostatic epithelial cells were lysed and fractionated using centrifugation into cytosolic/membrane and nuclear fractions. Proteins from lycopene-treated and placebo-treated cells were trypsinized and derivatized for quantitative proteomics using isobaric tags for relative and absolute quantitation (iTRAQ) reagent. Peptides were analyzed using two-dimensional microcapillary high-performance liquid chromatography-tandem mass spectrometry to identify proteins that were significantly upregulated or downregulated following lycopene exposure. Proteins that were most affected by lycopene were those involved in antioxidant responses, cytoprotection, apoptosis, growth inhibition, androgen receptor signaling, and the Akt/mTOR cascade. These data are consistent with previous studies suggesting that lycopene can prevent cancer in human prostatic epithelial cells at the stages of cancer initiation, promotion, and/or progression. (C) 2013 AACR.
引用
收藏
页码:419 / 427
页数:9
相关论文
共 50 条
[1]   Differential Protein Expression Analysis Using Stable Isotope Labeling and PQD Linear Ion Trap MS Technology [J].
Armenta, Jenny M. ;
Hoeschele, Ina ;
Lazar, Iulia M. .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2009, 20 (07) :1287-1302
[2]   Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome [J].
Beere, HM ;
Wolf, BB ;
Cain, K ;
Mosser, DD ;
Mahboubi, A ;
Kuwana, T ;
Tailor, P ;
Morimoto, RI ;
Cohen, GM ;
Green, DR .
NATURE CELL BIOLOGY, 2000, 2 (08) :469-475
[3]  
Ben-Dor A, 2005, MOL CANCER THER, V4, P177
[4]   Tomato sauce supplementation and prostate cancer: Lycopene accumulation and modulation of biomarkers of carcinogenesis [J].
Bowen, P ;
Chen, LW ;
Stacewicz-Sapuntzakis, M ;
Duncan, C ;
Sharifi, R ;
Ghosh, L ;
Kim, HS ;
Christov-Tzelkov, K ;
van Breemen, R .
EXPERIMENTAL BIOLOGY AND MEDICINE, 2002, 227 (10) :886-893
[5]   Oxidative DNA damage in prostate cancer patients consuming tomato sauce-based entrees as a whole-food intervention [J].
Chen, LW ;
Stacewicz-Sapuntzakis, M ;
Duncan, C ;
Sharifi, R ;
Ghosh, L ;
van Breemen, R ;
Ashton, D ;
Bowen, PE .
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2001, 93 (24) :1872-1879
[6]   Carotenoid action on the immune response [J].
Chew, BP ;
Park, JS .
JOURNAL OF NUTRITION, 2004, 134 (01) :257S-261S
[7]   LYCOPENE AS THE MOST EFFICIENT BIOLOGICAL CAROTENOID SINGLET OXYGEN QUENCHER [J].
DI MASCIO, P ;
KAISER, S ;
SIES, H .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 274 (02) :532-538
[8]   14-3-3 proteins interact with specific MEK kinases [J].
Fanger, GR ;
Widmann, C ;
Porter, AC ;
Sather, S ;
Johnson, GL ;
Vaillancourt, RR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (06) :3476-3483
[9]   Identification of a Cell of Origin for Human Prostate Cancer [J].
Goldstein, Andrew S. ;
Huang, Jiaoti ;
Guo, Changyong ;
Garraway, Isla P. ;
Witte, Owen N. .
SCIENCE, 2010, 329 (5991) :568-571
[10]   Systematic investigation of lycopene effects in LNCaP cells by use of novel large-scale proteomic analysis software [J].
Goo, Young Ah ;
Li, Zheng ;
Pajkovic, Natasa ;
Shaffer, Scott ;
Taylor, Greg ;
Chen, Jonzhi ;
Campbell, David ;
Arnstein, Larry ;
Goodlett, David R. ;
van Breemen, Richard B. .
PROTEOMICS CLINICAL APPLICATIONS, 2007, 1 (05) :513-523