Protein-monolayer engineering: principles and application to biocatalysis

被引：54

作者：

Nicolini, C ^{[1
]}

机构：

[1] ELBA FDN,MARCIANA MARINA,LI,ITALY

来源：

TRENDS IN BIOTECHNOLOGY | 1997年 / 15卷 / 10期

关键词：

D O I：

10.1016/S0167-7799(97)01084-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Highly ordered protein monolayers with defined structures and functions can be generated using appropriate modifications of the original Langmuir-Blodgett method. The characteristics of such monolayers of proteins, and enzymes in particular, are summarized, together with an evaluation of their potential applications to biocatalysis. None of the other immobilization techniques confers such long-term protection and thermostability on the structure and function of immobilized proteins.

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页码：395 / 401

页数：7

相关论文

共 44 条

[1] EFFECT OF TEMPERATURE ON OPTICAL-PROPERTIES OF REACTION CENTERS ORGANIZED IN LANGMUIR-BLODGETT-FILMS [J].

ANTOLINI, F ;

TROTTA, M ;

NICOLINI, C .

THIN SOLID FILMS, 1995, 254 (1-2) :252-256

[2] HEAT-STABLE LANGMUIR-BLODGETT-FILM OF GLUTATHIONE-S-TRANSFERASE [J].

ANTOLINI, F ;

PADDEU, S ;

NICOLINI, C .

LANGMUIR, 1995, 11 (07) :2719-2725

[3] POLAR LANGMUIR-BLODGETT FILMS. [J].

Blinov, L.M. ;

Dubinin, N.V. ;

Mikhnev, L.V. ;

Yudin, S.G. .

Thin Solid Films, 1984, 120 (03) :161-170

[4]

BRYCE MR, 1994, SCIENCE, V374, P771

[5] CONTROLLED TRANSFER OF EXCITATION ENERGY THROUGH THIN LAYERS [J].

BUCHER, H ;

DREXHAGE, KH ;

FLECK, M ;

KUHN, H ;

MOBIUS, D ;

SCHAFER, FP ;

SONDERMA.J ;

SPERLING, W ;

TILLMANN, P ;

WIEGAND, J .

MOLECULAR CRYSTALS, 1967, 2 (03) :199-&

[6]

Clair N. L. St., 1992, J AM CHEM SOC, V114, P7314

[7] REVERSED MICELLAR APPROACH AS A NEW TOOL FOR THE FORMATION AND STRUCTURAL STUDIES OF PROTEIN LANGMUIR-BLODGETT-FILMS [J].

EROKHIN, V ;

VAKULA, S ;

NICOLINI, C .

THIN SOLID FILMS, 1994, 238 (01) :88-94

[8] 2-DIMENSIONAL ORDER AND PROTEIN THERMAL-STABILITY - HIGH-TEMPERATURE PRESERVATION OF STRUCTURE AND FUNCTION [J].

EROKHIN, V ;

FACCI, P ;

NICOLINI, C .

BIOSENSORS & BIOELECTRONICS, 1995, 10 (1-2) :25-34

[9] CHEMICALLY-INDUCED ANISOTROPY IN ANTIBODY LANGMUIR-BLODGETT-FILMS [J].

FACCI, P ;

EROKHIN, V ;

ANTOLINI, F ;

NICOLINI, C .

THIN SOLID FILMS, 1994, 237 (1-2) :19-21

[10] SCANNING-TUNNELING-MICROSCOPY OF A MONOLAYER OF REACTION CENTERS [J].

FACCI, P ;

EROKHIN, V ;

NICOLINI, C .

THIN SOLID FILMS, 1994, 243 (1-2) :403-406

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