Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor

被引：6

作者：

Fulceri, R

Rossi, R

Bottinelli, R

Conti, A

Intravaia, E

Galione, A

Benedetti, A

Sorrentino, V

Reggiani, C

机构：

[1] Univ Padua, Dept Anat & Physiol, I-35131 Padua, Italy

[2] Univ Oxford, Dept Pharmacol, Oxford OX1 3QT, England

[3] Univ Siena, Dept Neurosci, Mol Med Sect, I-53100 Siena, Italy

[4] Ist Sci San Raffaele, DIBIT, Milan, Italy

[5] Univ Pavia, Inst Human Physiol, I-27100 Pavia, Italy

[6] Univ Siena, Dept Physiopathol & Expt Med, I-53100 Siena, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 288卷 / 03期

关键词：

skeletal muscles; cADP-ribose; ryanodine receptors; sarcoplasmic reticulum; single muscle fibers;

D O I：

10.1006/bbrc.2001.5834

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20% of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPM These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking. (C) 2001 Academic Press.

引用

页码：697 / 702

页数：6

共 32 条

[1]

Barone V, 1999, J MED GENET, V36, P115

[2] Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles [J].