Determination of trimetazidine HCl by adsorptive stripping square-wave voltammetry at a glassy carbon electrode

The adsorptive and electrochemical behavior of trimetazidine hydrochloride on a glassy carbon electrode were investigated in acetate buffer solution by using cyclic and square-wave voltammetry. Cyclic voltammetric studies indicated the oxidation of trimetazidine hydrochloride at the electrode surface through a single two-electron irreversible step and fundamentally controlled by adsorption. The solution condition and instrumental parameters were optimized for the determination of the authentic drug using adsorptive square wave stripping voltammetry. Trimetazidine hydrochloride gave a sensitive adsorptive oxidative peak at 0.750 V (vs. Ag/AgCl). The oxidation peak was used to determine authentic trimetazidine hydrochloride concentration in the range 5.0 x 10(-8)-5.0 x 10(-6) M with a detection limit of 2.0 x 10(-8) M. The procedure was successfully applied for assay of trimetazidine hydrochloride in the tablet dosage form (Vastarel). A mean recovery of 94.7% with a relative standard deviation (R.S.D.) of 0.88% was obtained. Applicability to assay the drug in urine samples was illustrated. The peak current was linear with the drug concentration in the range 17-85 mug per ml urine. The detection limit was 1.7 ug ml(-1) urine. (C) 2002 Elsevier Science B.V. All rights reserved.