Gene synthesis demystified

被引：106

作者：

Czar, Michael J. ^{[1
]}

Anderson, J. Christopher ^{[2
]}

Bader, Joel S. ^{[3
,4
]}

Peccoud, Jean ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA

[2] Univ Calif Berkeley, Dept Bioengn, Inst Quantitat Biol Res, Phys Biosci Div,Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

[3] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA

[4] Johns Hopkins Univ, High Throughput Biol Ctr, Baltimore, MD 21218 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2009年 / 27卷 / 02期

关键词：

LIGATION-INDEPENDENT CLONING; ENZYMATIC AMPLIFICATION; CHEMICAL-SYNTHESIS; ESCHERICHIA-COLI; DNA-SEQUENCES; IN-VITRO; DESIGN; RECOMBINATION; EXPRESSION; BIOLOGY;

D O I：

10.1016/j.tibtech.2008.10.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA fabrication of genetic cassettes at base-level precision is transforming genetic engineering from a laborious art to an information-driven discipline. Although substantial advances have been made in the development of DNA fabrication, the methods employed vary widely based on the length of the DNA. All of these methods are available commercially, but can also be performed at the molecular biology bench using typical reagents and procedures. Because the technology is not mature and is still evolving rapidly, it is helpful to gain some understanding of the different steps in this process and the associated technical challenges to successfully take advantage of DNA fabrication in a research project.

引用

页码：63 / 72

页数：10

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