Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

被引：264

作者：

Hofmann, Martin A. ^{[1
]}

Renzullo, Sandra ^{[1
]}

Mader, Markus ^{[1
]}

Chaignat, Valerie ^{[1
]}

Worwa, Gabriella ^{[1
]}

Thuer, Barbara ^{[1
]}

机构：

[1] Inst Virol & Immunoprophylaxis, CH-3147 Mittelhausern, Switzerland

来源：

EMERGING INFECTIOUS DISEASES | 2008年 / 14卷 / 12期

关键词：

D O I：

10.3201/eid1412.080818

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A novel bluetongue virus (BTV) termed Toggenburg orbivirus (TOV) was detected in goats from Switzerland by using real-time reverse transcription-PCR. cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplified by PCR and cloned into a plasmid vector. Five clones for each genome segment were sequenced to determine a consensus sequence. BLAST analysis and dendrogram construction showed that TOV is closely related to BTV, although some genome segments are distinct from the 24 known BTV serotypes. Maximal sequence identity to any BTV ranged from 63% (segment 2) to 79% (segments 7 and 10). Because the gene encoding outer capsid protein 2 (VP2), which determines the serotype of BTV, is placed within the BTV serogroup, we propose that TOV represents an unknown 25th serotype of BTV.

引用

页码：1855 / 1861

页数：7

共 23 条

[1] No evidence of bluetongue virus in Switzerland [J].

Cagienard, A. ;

Thuer, B. ;

Griot, C. ;

Hamblin, C. ;

Staerk, K. D. C. .

VETERINARY MICROBIOLOGY, 2006, 116 (1-3) :13-20

[2] EVOLUTION OF THE L2 GENE OF STRAINS OF BLUETONGUE VIRUS SEROTYPE-10 ISOLATED IN CALIFORNIA [J].

DEMATTOS, CCP ;

DEMATTOS, CA ;

OSBURN, BI ;

MACLACHLAN, NJ .

VIROLOGY, 1994, 201 (01) :173-177

[3] Bluetongue disease teaches Switzerland [J].

Hofmann, M. ;

Griot, C. ;

Chaignat, V. ;

Perler, L. ;

Thuer, B. .

SCHWEIZER ARCHIV FUR TIERHEILKUNDE, 2008, 150 (02) :49-56

[4] Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes [J].

Maan, S. ;

Maan, N. S. ;

Samuel, A. R. ;

Rao, S. ;

Attoui, H. ;

Mertens, P. P. C. .

JOURNAL OF GENERAL VIROLOGY, 2007, 88 :621-630

[5] Sequence analysis of bluetongue virus serotype 8 from the Netherlands 2006 and comparison to other European strains [J].

Maan, Sushila ;

Maan, Narender S. ;

Ross-Smith, Natalie ;

Batten, Carrie A. ;

Shaw, Andrew E. ;

Anthony, Simon J. ;

Samuel, Alan R. ;

Darpel, Karin E. ;

Veronesi, Eva ;

Oura, Chris A. L. ;

Singh, Karam P. ;

Nomikou, Kyriaki ;

Potgieter, Abraham C. ;

Attoui, Houssam ;

van Rooij, Eugene ;

van Rijn, Piet ;

De Clercq, Kris ;

Vandenbussche, Frank ;

Zientara, Stephan ;

Breard, Emmanuel ;

Sailleau, Corinne ;

Beer, Martin ;

Hoffman, Bernd ;

Mellor, Philip S. ;

Mertens, Peter P. C. .

VIROLOGY, 2008, 377 (02) :308-318

[6] Impact of bluetongue virus infection on the international movement and trade of ruminants [J].

MacLachlan, NJ ;

Osburn, BI .

JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2006, 228 (09) :1346-1349

[7] THE PATHOGENESIS AND IMMUNOLOGY OF BLUETONGUE VIRUS-INFECTION OF RUMINANTS [J].

MACLACHLAN, NJ .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 1994, 17 (3-4) :197-206

[8] First occurrence of Culicoides obsoletus-transmitted Bluetongue virus epidemic in Central Europe [J].

Mehlhorn, Heinz ;

Walldorf, Volker ;

Klimpel, Sven ;

Jahn, Birgit ;

Jaeger, Friedhelm ;

Eschweiler, Josef ;

Hoffmann, Bernd ;

Beer, Martin .

PARASITOLOGY RESEARCH, 2007, 101 (01) :219-228

[9]

MELLOR PS, 1995, ANN TROP MED PARASIT, V89, P1

[10] Bluetongue virus in the Mediterranean Basin 1998-2001 [J].

Mellor, PS ;

Wittmann, EJ .

VETERINARY JOURNAL, 2002, 164 (01) :20-37

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