Bioengineering and characterization of DNA-protein assemblies floating on supported membranes

被引：18

作者：

Boireau, W

Bombard, S

Sari, MA

Pompon, D ^{[1
]}

机构：

[1] CNRS, Ctr Genet Mol, Lab Ingn Prot Membranaires, F-91198 Gif Sur Yvette, France

[2] CNRS, UMR 8601, Chim & Biochim Pharmacol & Toxicol Lab, F-75006 Paris, France

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2002年 / 77卷 / 02期

关键词：

biosensor; hybrid bilayer; lateral mobility; redox protein; DNA chips; nanotechnology; plasmon resonance;

D O I：

10.1002/bit.10111

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip bio-sensors. (C) 2002 John Wiley Sons, Inc.

引用

页码：225 / 231

页数：7

共 25 条

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