Systematic genetic analysis with ordered arrays of yeast deletion mutants

被引：1562

作者：

Tong, AHY

Evangelista, M

Parsons, AB

Xu, H

Bader, GD

Pagé, N

Robinson, M

Raghibizadeh, S

Hogue, CWV

Bussey, H

Andrews, B

Tyers, M

Boone, C

机构：

[1] Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

[3] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada

[4] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[5] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Program Mol Biol & Canc, Toronto, ON M5G 1X5, Canada

[6] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada

[7] Virtek Engn Sci Inc, Toronto, ON M5R 2J7, Canada

来源：

SCIENCE | 2001年 / 294卷 / 5550期

关键词：

D O I：

10.1126/science.1065810

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Saccharomyces cerevisiae, more than 80% of the similar to 6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of similar to 4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization (BNI1, ARP2, ARC40, BIM1), DNA synthesis and repair (SGS1, RAD27), or uncharacterized functions (BBC1, NBP2) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

引用

页码：2364 / 2368

页数：5

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