Promotion of Sophora subprosrate polysaccharide on nitric oxide and interleukin-2 production in murine T lymphocytes:: implicated Ca2+ and protein kinase C

被引：17

作者：

Hu, TJ

Zheng, RL ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China

[2] Chinese Acad Agr Sci, Inst Tradit Chinese Vet Med, Lanzhou 730050, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2004年 / 4卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Sophora subprosrate polysaccharide; T lymphocytes; Ca2+; protein kinase C;

D O I：

10.1016/j.intimp.2003.11.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

SSP1, a polysaccharide isolated from Sophora subprosrate, increased the productions of nitric oxide (NO) and interleukin-2 (IL-2) in murine splenic T lymphocytes. SSP1-treated T lymphocytes showed an enhanced activity of protein kinase C (PKC) from the cellular membrane fraction as well as an increase in concentration of cytoplasmic free Ca2+. The results showed that SSP1 activated T cells to release NO and secrete IL-2 by modulating activity of PKC and level of intracellular free calcium. Nifedipine, a Ca2+ blocker, inhibited the activation of T cells by SSP1, indicating a role of Ca2+ in the activation of T cells. SSP1-treated T cells showed an enhanced translocation of PKC, indicating that SSP1 activated T cells via the activation of PKC. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：109 / 118

页数：10

共 38 条

[1]

ANA MG, 1998, CELL SIGNAL, V10, P721

[2]

[Anonymous], 2000, INSTANT NOTES IMMUNO

[3]

[Anonymous], GUANGXI MED

[4]

ASHOK K, 1997, INT J IMMUNOPHARMACO, V19, P129