Cryo-TEM studies of DNA and DNA-lipid structures

被引：26

作者：

Alfredsson, V ^{[1
]}

机构：

[1] Lund Univ, SE-22100 Lund, Sweden

来源：

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE | 2005年 / 10卷 / 5-6期

关键词：

cryo-TEM; DNA; oligonucleotide; lipid; lipoplex; transfection;

D O I：

10.1016/j.cocis.2005.09.005

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Studies published between 2001 and 2005 that utilise cryogenic transmission electron microscopy as a tool for investigating solutions containing DNA with or without amphiphiles present are reviewed. DNA or oligonucleotides form complexes with amphiphilic molecules such as lipids, so-called lipoplexes, and the structures and morphologies are excellent objects to study by cryogenic transmission electron microscopy. Recent studies show that this technique is in effective tool for identification of a range of structures such as unilamellar or multilamellar vesicles or dispersed liquid crystalline phases. (c) 2005 Elsevier Ltd. All rights reserved.

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页码：269 / 273

页数：5

相关论文

共 26 条

[1] Cryo-electron microscopy of vitreous sections of native biological cells and tissues [J].

Al-Amoudi, A ;

Norlen, LPO ;

Dubochet, J .

JOURNAL OF STRUCTURAL BIOLOGY, 2004, 148 (01) :131-135

[2] Cryo-electron microscopy of vitreous sections [J].

Al-Amoudi, A ;

Chang, JJ ;

Leforestier, A ;

McDowall, A ;

Salamin, LM ;

Norlén, LPO ;

Richter, K ;

Blanc, NS ;

Studer, D ;

Dubochet, J .

EMBO JOURNAL, 2004, 23 (18) :3583-3588

[3] Cryo transmission electron microscopy of liposomes and related structures [J].

Almgren, M ;

Edwards, K ;

Karlsson, G .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 174 (1-2) :3-21

[4] Cationic lipid-mediated transfection in vitro and in vivo [J].

Audouy, S ;

Hoekstra, D .

MOLECULAR MEMBRANE BIOLOGY, 2001, 18 (02) :129-143

[5] Stabilized lipid coated lipoplexes for the delivery of antisense oligonucleotides to liver endothelial cells in vitro and in vivo [J].

Bartsch, M ;

Weeke-Klimp, AH ;

Hoenselaar, EPD ;

Stuart, MCA ;

Meijer, DKF ;

Scherphof, GL ;

Kamps, JAAM .

JOURNAL OF DRUG TARGETING, 2004, 12 (9-10) :613-621

[6] Evidence for three-dimensional interlayer correlations in cationic lipid-DNA complexes as observed by cryo-electron microscopy [J].

Battersby, BJ ;

Grimm, R ;

Huebner, S ;

Cevc, G .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1372 (02) :379-383

[7] Freeze fracture direct imaging: A new freeze fracture method for specimen preparation in cryo-transmission electron microscopy [J].

Belkoura, L ;

Stubenrauch, C ;

Strey, R .

LANGMUIR, 2004, 20 (11) :4391-4399

[8] Transfection mediated by gemini surfactants: Engineered escape from the endosomal compartment [J].

Bell, PC ;

Bergsma, M ;

Dolbnya, IP ;

Bras, W ;

Stuart, MCA ;

Rowan, AE ;

Feiters, MC ;

Engberts, JBFN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (06) :1551-1558

[9] DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects [J].

Blanc, NS ;

Senn, A ;

Leforestier, A ;

Livolant, F ;

Dubochet, J .

JOURNAL OF STRUCTURAL BIOLOGY, 2001, 134 (01) :76-81

[10] Large-scale production of lipoplexes with long shelf-life [J].

Clement, J ;

Kiefer, K ;

Kimpfler, A ;

Garidel, P ;

Peschka-Süss, R .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2005, 59 (01) :35-43