UPLC-TOF-MS for plant metabolomics:: A sequential approach for wound marker analysis in Arabidopsis thaliana

被引：86

作者：

Grata, Elia ^{[1
,2
]}

Boccard, Julien ^{[1
,3
]}

Guillarme, Davy ^{[1
]}

Glauser, Gaetan ^{[1
,2
]}

Carrupt, Pierre-Alain ^{[3
]}

Farmer, Edward E. ^{[4
]}

Wolfender, Jean-Luc ^{[2
]}

Rudaz, Serge ^{[1
]}

机构：

[1] Univ Lausanne, Univ Geneva, EPGL, Sch Pharmaceut Sci,Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland

[2] Univ Lausanne, Univ Geneva, EPGL, Sch Pharmaceut Sci,Lab Pharmacognosy & Phytochem, CH-1211 Geneva 4, Switzerland

[3] Univ Lausanne, Univ Geneva, EPGL, Sch Pharmaceut Sci,LCT Pharmacochem, CH-1211 Geneva 4, Switzerland

[4] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 871卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

UPLC-TOF-MS; Arabidopsis thaliana; metabolomics; signalling; oxylipins;

D O I：

10.1016/j.jchromb.2008.04.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The model plant Arabidopsis thaliana was studied for the search of new metabolites involved in wound signalling. Diverse LC approaches were considered in terms of efficiency and analysis time and a 7-min gradient on a UPLC-TOF-MS system with a short column was chosen for metabolite fingerprinting. This screening step was designed to allow the comparison of a high number of samples over a wide range of time points after stress induction in positive and negative ionisation modes. Thanks to data treatment, clear discrimination was obtained, providing lists of potential stress-induced ions. in a second step, the fingerprinting conditions were transferred to longer column, providing a higher peak capacity able to demonstrate the presence of isomers among the highlighted compounds. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：261 / 270

页数：10

共 46 条

[1] Multivariate data analysis of rapid LC-TOF/MS experiments from Arabidopsis thaliana stressed by wounding [J].