Phosphorylation of neurofibromatosis type 1 gene product (neurofibromin) by cAMP-dependent protein kinase

被引：55

作者：

Izawa, I

Tamaki, N

Saya, H

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT NEUROONCOL,HOUSTON,TX

[2] KOBE UNIV,SCH MED,DEPT NEUROSURG,KOBE 650,JAPAN

[3] KUMAMOTO UNIV,SCH MED,DEPT TUMOR GENET & BIOL,KUMAMOTO 860,JAPAN

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

neurofibromatosis type 1 (NF1); neurofibromin; phosphorylation; cAMP-dependent protein kinase;

D O I：

10.1016/0014-5793(96)00137-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The critical function of the neurofibromatosis type 1 (NF1) gene product (neurofibromin) is not well defined except that neurofibromin has homology with a family of the GTPase-activating proteins (GAPs), In this study, we confirmed that neurofibromin is constitutively phosphorylated and detected kinase activities which specifically phosphorylate the cystein/serine-rich domain and the C-terminal domain of the neurofibromin in cell lysate, In vitro and in-gel kinase assays strongly indicated that cAMP-dependent protein kinase (PKA) is a candidate for the neurofibromin kinase, The biological significance of the phosphorylation of neurofibromin is unclear at present, but we speculate that neurofibromin plays a crucial role in cellular function since it links the two major cellular pathways which are the GAP-ras and PKA-associated signals.

引用

页码：53 / 59

页数：7

共 43 条

[1] A CONSERVED ALTERNATIVE SPLICE IN THE VONRECKLINGHAUSEN NEUROFIBROMATOSIS (NF1) GENE PRODUCES 2 NEUROFIBROMIN ISOFORMS, BOTH OF WHICH HAVE GTPASE-ACTIVATING PROTEIN-ACTIVITY [J].