Single DNA sequence common to all chlamydial species employed for PCR detection of these organisms

被引:7
作者
Girjes, AA
Carrick, FN
Lavin, MF
机构
[1] Queensland Inst Med Res, Queensland Canc Fund, Res Unit, Bancroft Ctr, Brisbane, Qld 4029, Australia
[2] Univ Queensland, Dept Anat Sci, Brisbane, Qld 4072, Australia
[3] Univ Queensland, Dept Surg, Brisbane, Qld 4072, Australia
[4] Univ Queensland, Dept Zool & Entomol, Brisbane, Qld 4072, Australia
关键词
Chlamydia; PCR; pathogen detection; universal primers;
D O I
10.1016/S0923-2508(99)00108-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chlamydial infection is responsible for a wide spectrum of diseases of the eye, genitourinary tract, and lung. This group of organisms is also implicated in the pathogenesis of coronary artery disease as well as arthritis. Since cross-species infection is widely reported (though probably underestimated), it is an advantage to have a rapid and reliable method to detect all forms of chlamydiae in patient samples. We have identified a 160/163-bp DNA fragment in Chlamydia which is highly conserved in all chlamydial species. A polymerase chain reaction method based on this sequence has been developed to detect, in clinical samples, chlamydiae which have been shown to be positive by fluorescent-staining immunoassay; this method can be utilized in combination with restriction endonuclease cleavage to identify individual chlamydial species. Thus we have developed a sensitive and rapid detection method and have used it on samples from patients with respiratory and genital infections. (C) 1999 Editions scientifiques et medicales Elsevier SAS.
引用
收藏
页码:483 / 489
页数:7
相关论文
共 22 条
[1]  
BELUNIS CJ, 1992, J BIOL CHEM, V267, P18702
[2]   DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS EYE INFECTION IN TANZANIA BY POLYMERASE CHAIN-REACTION ENZYME-IMMUNOASSAY [J].
BOBO, L ;
MUNOZ, B ;
VISCIDI, R ;
QUINN, T ;
MKOCHA, H ;
WEST, S .
LANCET, 1991, 338 (8771) :847-850
[3]   ISOLATION AND TYPING OF A STRAIN OF CHLAMYDIA-PSITTACI FROM ANGORA GOATS [J].
BROWN, AS ;
AMOS, ML ;
LAVIN, MF ;
GIRJES, AA ;
TIMMS, P ;
WOOLCOCK, JB .
AUSTRALIAN VETERINARY JOURNAL, 1988, 65 (09) :288-289
[4]   DETECTION OF CHLAMYDIA-TRACHOMATIS IN CLINICAL SPECIMENS BY THE POLYMERASE CHAIN-REACTION [J].
CLAAS, HC ;
MELCHERS, WJ ;
DEBRUIJN, IH ;
DEGRAAF, M ;
VANDIJK, WC ;
LINDEMAN, J ;
QUINT, WG .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1990, 9 (12) :864-868
[5]   Diagnosis of ornithosis by cell culture and polymerase chain reaction in a patient with chronic pneumonia [J].
Essig, A ;
Zucs, P ;
Susa, M ;
Wasenauer, G ;
Mamat, U ;
Hetzel, M ;
Vogel, U ;
Wieshammer, S ;
Brade, H ;
Marre, R .
CLINICAL INFECTIOUS DISEASES, 1995, 21 (06) :1495-1497
[6]   PULSED-FIELD GEL-ELECTROPHORESIS DETERMINATION OF THE GENOME SIZE OF OBLIGATE INTRACELLULAR BACTERIA BELONGING TO THE GENERA CHLAMYDIA, RICKETTSIELLA, AND POROCHLAMYDIA [J].
FRUTOS, R ;
PAGES, M ;
BELLIS, M ;
ROIZES, G ;
BERGOIN, M .
JOURNAL OF BACTERIOLOGY, 1989, 171 (08) :4511-4513
[7]  
Fryer RH, 1997, J INVEST MED, V45, P168
[8]   PROPOSAL OF CHLAMYDIA-PECORUM SP-NOV FOR CHLAMYDIA STRAINS DERIVED FROM RUMINANTS [J].
FUKUSHI, H ;
HIRAI, K .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (02) :306-308
[9]   REMARKABLE SEQUENCE RELATEDNESS IN THE DNA ENCODING THE MAJOR OUTER-MEMBRANE PROTEIN OF CHLAMYDIA-PSITTACI (KOALA TYPE-I) AND CHLAMYDIA-PNEUMONIA [J].
GIRJES, AA ;
CARRICK, FN ;
LAVIN, MF .
GENE, 1994, 138 (1-2) :139-142
[10]   2 DISTINCT FORMS OF CHLAMYDIA-PSITTACI ASSOCIATED WITH DISEASE AND INFERTILITY IN PHASCOLARCTOS-CINEREUS (KOALA) [J].
GIRJES, AA ;
HUGALL, AF ;
TIMMS, P ;
LAVIN, MF .
INFECTION AND IMMUNITY, 1988, 56 (08) :1897-1900