A cell-culture system for long-term maintenance of elevated hydrostatic pressure with the option of additional tension

被引：21

作者：

Hasel, C ^{[1
]}

Dürr, S ^{[1
]}

Brüderlein, S ^{[1
]}

Melzner, I ^{[1
]}

Möller, P ^{[1
]}

机构：

[1] Univ Ulm, Dept Pathol, D-89089 Ulm, Germany

来源：

JOURNAL OF BIOMECHANICS | 2002年 / 35卷 / 05期

关键词：

cellular stress; hydrostatic pressure; pressurized chamber;

D O I：

10.1016/S0021-9290(01)00237-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In cell stress research, there is still a need to apply long-term hydrostatic pressure without changing any other environmental condition. We present here a new, open, pressurized chamber system allowing long-term sustained and dynamic application of hydrostatic pressure with the option of additional tension. Based on the computer-controlled Flexcell Strain Unit, we designed a pressurized chamber with a dynamic airflow and a defined membrane extension, which can be regulated by spacers. During operation up to 26.6 kPa, O-2 partial pressures and pH in the cell-culture medium do not change compared to control cultures kept at normal atmosphere. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：579 / 584

页数：6

共 15 条

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