Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes

被引：140

作者：

Chen, Yu ^{[1
]}

Wang, Yan ^{[3
]}

Zhang, Jinzhong ^{[3
]}

Deng, Yongqiang ^{[3
]}

Jiang, Li ^{[3
]}

Song, Eli ^{[3
]}

Wu, Xufeng S. ^{[2
]}

Hammer, John A. ^{[2
]}

Xu, Tao ^{[3
]}

Lippincott-Schwartz, Jennifer ^{[1
]}

机构：

[1] Eugene Kennedy Shriver Natl Inst Child Hlth & Hum, Cell Biol & Metab Program, NIH, Bethesda, MD 20892 USA

[2] NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA

[3] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

来源：

JOURNAL OF CELL BIOLOGY | 2012年 / 198卷 / 04期

基金：

美国国家科学基金会; 北京市自然科学基金;

关键词：

GTPASE-ACTIVATING-PROTEIN; GLUCOSE-TRANSPORTER GLUT4; REGULATED TRANSPORT; 3T3-L1; ADIPOCYTES; MEMBRANE; TRAFFICKING; AS160; GLUCOSE-TRANSPORTER-4; EXOCYTOSIS; REQUIRES;

D O I：

10.1083/jcb.201111091

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Rab proteins are important regulators of insulin-stimulated GLUT4 translocation to the plasma membrane (PM), but the precise steps in GLUT4 trafficking modulated by particular Rab proteins remain unclear. Here, we systematically investigate the involvement of Rab proteins in GLUT4 trafficking, focusing on Rab proteins directly mediating GLUT4 storage vesicle (GSV) delivery to the PM. Using dual-color total internal reflection fluorescence (TIRF) microscopy and an insulin-responsive aminopeptidase (IRAP)-pHluorin fusion assay, we demonstrated that Rab10 directly facilitated GSV translocation to and docking at the PM. Rab14 mediated GLUT4 delivery to the PM via endosomal compartments containing transferrin receptor (TfR), whereas Rab4A, Rab4B, and Rab8A recycled GLUT4 through the endosomal system. Myosin-Va associated with GSVs by interacting with Rab10, positioning peripherally recruited GSVs for ultimate fusion. Thus, multiple Rab proteins regulate the trafficking of GLUT4, with Rab10 coordinating with myosin-Va to mediate the final steps of insulin-stimulated GSV translocation to the PM.

引用

页码：545 / 560

页数：16

共 42 条

[1]

Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells [J].