Rice straw as a support for immobilization of microbial lipase

被引：31

作者：

de Castro, HF

de Lima, R

Roberto, IC

机构：

[1] Fac Chem Engn Lorena, Dept Chem Engn, BR-12600970 Lorena, SP, Brazil

[2] Fac Chem Engn Lorena, Dept Biotechnol, BR-12600970 Lorena, SP, Brazil

来源：

BIOTECHNOLOGY PROGRESS | 2001年 / 17卷 / 06期

关键词：

D O I：

10.1021/bp010099t

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2(2) factorial design. Higher immobilization yields (> 70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.

引用

收藏

页码：1061 / 1064

页数：4

相关论文

共 14 条

[1] Fermentation of xylose and rice straw hydrolysate to ethanol by Candida shehatae NCL-3501 [J].

Abbi, M ;

Kuhad, RC ;

Singh, A .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1996, 17 (01) :20-23

[2] Bioreactors with immobilized lipases: State of the art [J].

Balcao, VM ;

Paiva, AL ;

Malcata, FX .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (06) :392-416

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] ETHANOL-PRODUCTION BY WHOLE CELL IMMOBILIZATION USING LIGNOCELLULOSIC MATERIALS AS SOLID MATRIX [J].

DAS, D ;

GAIDHANI, NR ;

MURARI, K ;

GUPTA, PS .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1993, 75 (02) :132-137

[5] Immobilization of porcine pancreatic lipase on celite for application in the synthesis of butyl butyrate in a nonaqueous system [J].

de Castro, HF ;

de Oliveira, PC ;

Soares, CMF ;

Zanin, GM .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1999, 76 (01) :147-152

[6] Applications of lipase [J].

Gandhi, NN .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1997, 74 (06) :621-634

[7] Microbial lipases form versatile tools for biotechnology [J].

Jaeger, KE ;

Reetz, MT .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (09) :396-403

[8] Physical characterization of pulps .1. Rice straw bleached by nonconventional multistage method and paper sheet making [J].

Mansour, OY ;

Selim, IZ ;

Mohamed, SA .

POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, 1996, 35 (04) :567-580

[9] XYLITOL PRODUCTION BY CANDIDA-GUILLIERMONDII AS AN APPROACH FOR THE UTILIZATION OF AGROINDUSTRIAL RESIDUES [J].

ROBERTO, IC ;

FELIPE, MGA ;

DEMANCILHA, IM ;

VITOLO, M ;

SATO, S ;

DASILVA, SS .

BIORESOURCE TECHNOLOGY, 1995, 51 (2-3) :255-257

[10] Effects of additives on the activity of a covalently immobilised lipase in organic media [J].

Rocha, JMS ;

Gil, MH ;

Garcia, FAP .

JOURNAL OF BIOTECHNOLOGY, 1998, 66 (01) :61-67