SARS-CoV-2 infection protects against rechallenge in rhesus macaques

被引：656

作者：

Chandrashekar, Abishek ^{[1
]}

Liu, Jinyan ^{[1
]}

Martinot, Amanda J. ^{[1
,2
]}

McMahan, Katherine ^{[1
]}

Mercado, Noe B. ^{[1
]}

Peter, Lauren ^{[1
]}

Tostanoski, Lisa H. ^{[1
]}

Yu, Jingyou ^{[1
]}

Maliga, Zoltan ^{[3
]}

Nekorchuk, Michael ^{[4
]}

Busman-Sahay, Kathleen ^{[4
]}

Terry, Margaret ^{[4
]}

Wrijil, Linda M. ^{[2
]}

Ducat, Sarah ^{[2
]}

Martinez, David R. ^{[5
]}

Atyeo, Caroline ^{[3
,6
,7
]}

Fischinger, Stephanie ^{[6
,7
]}

Burke, John S. ^{[6
,7
]}

Slein, Matthew D. ^{[6
,7
]}

Pessaint, Laurent ^{[8
]}

Van Ry, Alex ^{[8
]}

Greenhouse, Jack ^{[8
]}

Taylor, Tammy ^{[8
]}

Blade, Kelvin ^{[8
]}

Cook, Anthony ^{[8
]}

Finneyfrock, Brad ^{[8
]}

Brown, Renita ^{[8
]}

Teow, Elyse ^{[8
]}

Velasco, Jason ^{[8
]}

Zahn, Roland ^{[9
]}

Wegmann, Frank ^{[9
]}

Abbink, Peter ^{[1
]}

Bondzie, Esther A. ^{[1
]}

Dagotto, Gabriel ^{[1
,3
]}

Gebre, Makda S. ^{[1
,3
]}

He, Xuan ^{[1
]}

Jacob-Dolan, Catherine ^{[1
,3
]}

Kordana, Nicole ^{[1
]}

Li, Zhenfeng ^{[1
]}

Lifton, Michelle A. ^{[1
]}

Mahrokhian, Shant H. ^{[1
]}

Maxfield, Lori F. ^{[1
]}

Nityanandam, Ramya ^{[1
]}

Nkolola, Joseph P. ^{[1
]}

Schmidt, Aaron G. ^{[6
,7
,10
]}

Miller, Andrew D. ^{[11
]}

Baric, Ralph S. ^{[5
]}

Alter, Galit ^{[6
,7
,10
]}

Sorger, Peter K. ^{[3
]}

Estes, Jacob D. ^{[4
]}

机构：

[1] Harvard Med Sch, Beth Israel Deaconess Med Ctr, Ctr Virol & Vaccine Res, Boston, MA 02215 USA

[2] Tufts Univ, Cummings Sch Vet Med, North Grafton, MA 01536 USA

[3] Harvard Med Sch, Boston, MA 02115 USA

[4] Oregon Hlth & Sci Univ, Beaverton, OR 97006 USA

[5] Univ North Carolina, Chapel Hill, NC 27599 USA

[6] MIT, Ragon Inst MGH, Cambridge, MA 02139 USA

[7] Harvard, Cambridge, MA 02139 USA

[8] Bioqual, Rockville, MD 20852 USA

[9] Janssen Vaccines & Prevent BV, Leiden, Netherlands

[10] Massachusetts Consortium Pathogen Readiness, Boston, MA 02215 USA

[11] Cornell Univ, Coll Vet Med, Ithaca, NY 14853 USA

来源：

SCIENCE | 2020年 / 369卷 / 6505期

基金：

比尔及梅琳达.盖茨基金会; 美国国家卫生研究院;

关键词：

REVERSE GENETICS; IMMUNITY; CDNA; SARS;

D O I：

10.1126/science.abc4776

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

An understanding of protective immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for vaccine and public health strategies aimed at ending the global coronavirus disease 2019 (COVID-19) pandemic. A key unanswered question is whether infection with SARS-CoV-2 results in protective immunity against reexposure. We developed a rhesus macaque model of SARS-CoV-2 infection and observed that macaques had high viral loads in the upper and lower respiratory tract, humoral and cellular immune responses, and pathologic evidence of viral pneumonia. After the initial viral clearance, animals were rechallenged with SARS-CoV-2 and showed 5 log(10) reductions in median viral loads in bronchoalveolar lavage and nasal mucosa compared with after the primary infection. Anamnestic immune responses after rechallenge suggested that protection was mediated by immunologic control. These data show that SARS-CoV-2 infection induced protective immunity against reexposure in nonhuman primates.

引用

页码：812 / +

页数：37

共 20 条

[1]

HIV-1 superinfection despite broad CD8+ T-cell responses containing replication of the primary virus [J].

Altfeld, M ;

Allen, TM ;

Yu, XG ;

Johnston, MN ;

Agrawal, D ;

Korber, BT ;

Montefiori, DC ;

O'Connor, DH ;

Davis, BT ;

Lee, PK ;

Maier, EL ;

Harlow, J ;

Goulder, PJR ;

Brander, C ;

Rosenberg, ES ;

Walker, BD .

NATURE, 2002, 420 (6914) :434-439

[2]

THE TIME COURSE OF THE IMMUNE-RESPONSE TO EXPERIMENTAL CORONAVIRUS INFECTION OF MAN [J].

CALLOW, KA ;

PARRY, HF ;

SERGEANT, M ;

TYRRELL, DAJ .

EPIDEMIOLOGY AND INFECTION, 1990, 105 (02) :435-446

[3]

A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster [J].

Chan, Jasper Fuk-Woo ;

Yuan, Shuofeng ;

Kok, Kin-Hang ;

To, Kelvin Kai-Wang ;

Chu, Hin ;

Yang, Jin ;

Xing, Fanfan ;

Liu, Jieling ;

Yip, Cyril Chik-Yan ;

Poon, Rosana Wing-Shan ;

Tsoi, Hoi-Wah ;

Lo, Simon Kam-Fai ;

Chan, Kwok-Hung ;

Poon, Vincent Kwok-Man ;

Chan, Wan-Mui ;

Ip, Jonathan Daniel ;

Cai, Jian-Piao ;

Cheng, Vincent Chi-Chung ;

Chen, Honglin ;

Hui, Christopher Kim-Ming ;

Yuen, Kwok-Yung .

LANCET, 2020, 395 (10223) :514-523

[4]

Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study [J].

Chen, Nanshan ;

Zhou, Min ;

Dong, Xuan ;

Qu, Jieming ;

Gong, Fengyun ;

Han, Yang ;

Qiu, Yang ;

Wang, Jingli ;

Liu, Ying ;

Wei, Yuan ;

Xia, Jia'an ;

Yu, Ting ;

Zhang, Xinxin ;

Zhang, Li .

LANCET, 2020, 395 (10223) :507-513

[5]

Dissecting Polyclonal Vaccine-Induced Humoral Immunity against HIV Using Systems Serology [J].

Chung, Amy W. ;

Kumar, Manu P. ;

Arnold, Kelly B. ;

Yu, Wen Han ;

Schoen, Matthew K. ;

Dunphy, Laura J. ;

Suscovich, Todd J. ;

Frahm, Nicole ;

Linde, Caitlyn ;

Mahan, Alison E. ;

Hoffner, Michelle ;

Streeck, Hendrik ;

Ackerman, Margaret E. ;

McElrath, M. Juliana ;

Schuitemaker, Hanneke ;

Pau, Maria G. ;

Baden, Lindsey R. ;

Kim, Jerome H. ;

Michael, Nelson L. ;

Barouch, Dan H. ;

Lauffenburger, Douglas A. ;

Alter, Galit .

CELL, 2015, 163 (04) :988-998

[6]

Impact of early cART in the gut during acute HIV infection [J].

Deleage, Claire ;

Schuetz, Alexandra ;

Alvord, W. Gregory ;

Johnston, Leslie ;

Hao, Xing-Pei ;

Morcock, David R. ;

Rerknimitr, Rungsun ;

Fletcher, James L. K. ;

Puttamaswin, Suwanna ;

Phanuphak, Nittaya ;

Dewar, Robin ;

McCune, Joseph M. ;

Sereti, Irini ;

Robb, Merlin ;

Kim, Jerome H. ;

Schacker, Timothy W. ;

Hunt, Peter ;

Lifson, Jeffrey D. ;

Ananworanich, Jintanat ;

Estes, Jacob D. .

JCI INSIGHT, 2016, 1 (10)

[7]

Deleage Claire, 2016, Pathog Immun, V1, P68

[8]

Multisystem Inflammatory Syndrome in US Children and Adolescents [J].

Feldstein, Leora R. ;

Rose, Erica B. ;

Horwitz, Steven M. ;

Collins, Jennifer P. ;

Newhams, Margaret M. ;

Son, Mary Beth F. ;

Newburger, Jane W. ;

Kleinman, Lawrence C. ;

Heidemann, Sabrina M. ;

Martin, Amarilis A. ;

Singh, Aalok R. ;

Li, Simon ;

Tarquinio, Keiko M. ;

Jaggi, Preeti ;

Oster, Matthew E. ;

Zackai, Sheemon P. ;

Gillen, Jennifer ;

Ratner, Adam J. ;

Walsh, Rowan F. ;

Fitzgerald, Julie C. ;

Keenaghan, Michael A. ;

Alharash, Hussam ;

Doymaz, Sule ;

Clouser, Katharine N. ;

Giuliano, John S. ;

Gupta, Anjali ;

Parker, Robert M. ;

Maddux, Aline B. ;

Havalad, Vinod ;

Ramsingh, Stacy ;

Bukulmez, Hulya ;

Bradford, Tamara T. ;

Smith, Lincoln S. ;

Tenforde, Mark W. ;

Carroll, Christopher L. ;

Riggs, Becky J. ;

Gertz, Shira J. ;

Daube, Ariel ;

Lansell, Amanda ;

Coronado Munoz, Alvaro ;

Hobbs, Charlotte V. ;

Marohn, Kimberly L. ;

Halasa, Natasha B. ;

Patel, Manish M. ;

Randolph, Adrienne G. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (04) :334-346

[9]

First Case of 2019 Novel Coronavirus in the United States [J].

Holshue, Michelle L. ;

DeBolt, Chas ;

Lindquist, Scott ;

Lofy, Kathy H. ;

Wiesman, John ;

Bruce, Hollianne ;

Spitters, Christopher ;

Ericson, Keith ;

Wilkerson, Sara ;

Tural, Ahmet ;

Diaz, George ;

Cohn, Amanda ;

Fox, LeAnne ;

Patel, Anita ;

Gerber, Susan I. ;

Kim, Lindsay ;

Tong, Suxiang ;

Lu, Xiaoyan ;

Lindstrom, Steve ;

Pallansch, Mark A. ;

Weldon, William C. ;

Biggs, Holly M. ;

Uyeki, Timothy M. ;

Pillai, Satish K. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 382 (10) :929-936

[10]

Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China [J].

Huang, Chaolin ;

Wang, Yeming ;

Li, Xingwang ;

Ren, Lili ;

Zhao, Jianping ;

Hu, Yi ;

Zhang, Li ;

Fan, Guohui ;

Xu, Jiuyang ;

Gu, Xiaoying ;

Cheng, Zhenshun ;

Yu, Ting ;

Xia, Jiaan ;

Wei, Yuan ;

Wu, Wenjuan ;

Xie, Xuelei ;

Yin, Wen ;

Li, Hui ;

Liu, Min ;

Xiao, Yan ;

Gao, Hong ;

Guo, Li ;

Xie, Jungang ;

Wang, Guangfa ;

Jiang, Rongmeng ;

Gao, Zhancheng ;

Jin, Qi ;

Wang, Jianwei ;

Cao, Bin .

LANCET, 2020, 395 (10223) :497-506

← 1 2 →