Electron cryo-microscopy of vitrified bulk biological specimens: Ideal and real structures of water-lipid phases

被引：13

作者：

Erk, I ^{[1
]}

Michel, M ^{[1
]}

Lepault, J ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,ELECT MICROSCOPY LAB 1,CH-8092 ZURICH,SWITZERLAND

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1996年 / 182卷

关键词：

electron cryo-microscopy; X-ray cryo-diffraction; cryo-sectioning; vitrification; high-pressure freezing; lipid-water phases; lipid lamellar structure; membrane defects;

D O I：

10.1111/j.1365-2818.1996.tb04793.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Lipid-water mixtures were studied by X-ray cryo-diffraction in order to assess the structural changes during freezing. We show that the water of aqueous lipid phases, in the concentration range of 10-30% (water weight/total weight), is vitrified by high-pressure freezing. Vitrified lipid phases can be cryo-sectioned and imaged by electron cryomicroscopy. Both the ideal or average and the real or local structures of the lipid mixtures can be studied at a resolution better than 2 nm. While the average structure of the lipid phases is in good agreement with that determined by X-ray diffraction, the local structure reveals features that might play an important role in the function of biological membranes such as in endo- and exocytosis.

引用

页码：15 / 23

页数：9

共 16 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].

ADRIAN, M ;

DUBOCHET, J ;

LEPAULT, J ;

MCDOWALL, AW .

NATURE, 1984, 308 (5954) :32-36

[2] CORRELATED X-RAY-DIFFRACTION AND FREEZE-FRACTURE STUDIES ON MEMBRANE MODEL SYSTEMS PERTURBATIONS INDUCED BY FREEZE-FRACTURE PREPARATIVE PROCEDURES [J].

COSTELLO, MJ ;

GULIKKRZYWICKI, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 455 (02) :412-432

[3] LOW-TEMPERATURE FORMS OF ICE AS STUDIED BY X-RAY DIFFRACTION [J].

DOWELL, LG ;

RINFRET, AP .

NATURE, 1960, 188 (4757) :1144-1148

[4] ELECTRON-MICROSCOPY OF FROZEN WATER AND AQUEOUS-SOLUTIONS [J].

DUBOCHET, J ;

LEPAULT, J ;

FREEMAN, R ;

BERRIMAN, JA ;

HOMO, JC .

JOURNAL OF MICROSCOPY, 1982, 128 (DEC) :219-237

[5] NEW INSTRUMENTS WHICH FACILITATE RAPID FREEZING AT 83-K AND 6-K [J].

ESCAIG, J .

JOURNAL OF MICROSCOPY, 1982, 126 (JUN) :221-229

[6] USE OF LOW-TEMPERATURE X-RAY-DIFFRACTION TO EVALUATE FREEZING METHODS USED IN FREEZE-FRACTURE ELECTRON-MICROSCOPY [J].

GULIKKRZYWICKI, T ;

COSTELLO, MJ .

JOURNAL OF MICROSCOPY, 1978, 112 (JAN) :103-113

[7]

JANIAK MJ, 1979, J BIOL CHEM, V254, P6068

[8] PROJECTED STRUCTURE OF UNSTAINED, FROZEN-HYDRATED T-LAYER OF BACILLUS-BREVIS [J].

LEPAULT, J ;

PITT, T .

EMBO JOURNAL, 1984, 3 (01) :101-105

[9] CRYO-ELECTRON MICROSCOPY OF ARTIFICIAL BIOLOGICAL-MEMBRANES [J].

LEPAULT, J ;

PATTUS, F ;

MARTIN, N .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 820 (02) :315-318

[10] LIPID PHASES - STRUCTURE AND STRUCTURAL TRANSITIONS [J].

LUZZATI, V ;

TARDIEU, A .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1974, 25 :79-94

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