Characterization of a Pinus pinaster cDNA encoding an auxin up-regulated putative peroxidase in roots

被引:12
作者
Charvet-Candela, V [1 ]
Hitchin, S [1 ]
Reddy, MS [1 ]
Cournoyer, B [1 ]
Marmeisse, R [1 ]
Gay, G [1 ]
机构
[1] Univ Lyon 1, UMR CNRS 5557, Lab Ecol Microbienne, F-69622 Villeurbanne, France
关键词
differential screening; gene expression; mycorrhiza;
D O I
10.1093/treephys/22.4.231
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
As part of a study to identify host plant genes regulated by fungal auxin during ectomycorrhiza formation, we differentially screened a cDNA library constructed from roots of auxin-treated Pinus pinaster (Ait.) Sol. seedlings. We identified three cDNAs up-regulated by auxin. Sequence analysis of one of these cDNAs, PpPrx75, revealed the presence of an open reading frame of 216 amino acids with the characteristic consensus sequences of plant peroxidases. The deduced amino acid sequence showed homology with Arabidopsis thaliana (L.) Heynh., Arachis hypogaea L. and Stylosanthes humilis HBK cationic peroxidases. Amino acid sequence identities in the conserved domains of plant peroxidases ranged from 60 to 100%. In PpPrx75, there are five cysteine residues and one histidine residue that are found at conserved positions among other peroxidases. A potential glycosylation site (NTS) is present in the deduced sequence. Phylogenetic analysis showed that PpPrx75 is closely related to two A. thaliana peroxidases. The PpPrx75 cDNA was induced by active auxins, ethylene, abscisic acid and quercetin, a flavonoid possibly involved in plant-microorganism interactions. Transcript accumulation was detected within 3 h following root induction by auxin, and the amount of mRNA increased over the following 24 h. The protein synthesis inhibitor cycloheximide did not inhibit indole-3-acetic acid-induced transcript accumulation, suggesting that PpPrx75 induction is a primary (direct) response to auxin. This cDNA can be used to study expression of an auxin-regulated peroxidase during ectomycorrhiza formation.
引用
收藏
页码:231 / 238
页数:8
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