Iodination of proteins by IPy2BF4, a new tool in protein chemistry

Iodination is a very useful method for protein characterization and labeling. However, derivatization chemistries used in most conventional iodination procedures may cause substantial alterations in protein structure and function. The IPy2BF4 reagent [ bis( pyridine) iodonium ( I) tetrafluoroborate] has been shown to be an effective iodinating reagent for peptides. Herein we report the first application of IPy2BF4 in protein iodination in an aqueous medium using three representative substrates: insulin, lysozyme, and the enzyme 1,3- 1,4-beta-(D)-4- glucanohydrolase. Our results show that IPy2BF4 has clear advantages over existing methods in that the reaction is quantitative, fast, and selective for the most accessible Tyr residues of a protein, and it preserves the functional integrity of the protein when moderate Tyr labeling levels are pursued.