The structure of the efflux pump AcrB in complex with bile acid

被引：50

作者：

Drew, David ^{[1
,2
]}

Klepsch, Mirjam M. ^{[3
]}

Newstead, Simon ^{[1
,2
]}

Flaig, Ralf ^{[4
]}

De Gier, Jan-Willem ^{[3
]}

Iwata, So ^{[1
,2
,4
,5
]}

Beis, Konstantinos ^{[1
,2
,4
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Membrane Prot Crystallog Grp, Div Mol Biosci, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Membrane Prot Lab, London SW7 2AZ, England

[3] Stockholm Univ, Dept Biochem & Biophys, Ctr Biomembrane Res, S-10691 Stockholm, Sweden

[4] Diamond Light Source, Chilton, Oxon, England

[5] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshida Konoe, Japan

来源：

MOLECULAR MEMBRANE BIOLOGY | 2008年 / 25卷 / 08期

基金：

瑞典研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

Multidrug efflux protein; AcrB; bile acid; E; coli; structure;

D O I：

10.1080/09687680802552257

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated primarily by the multidrug efflux pump, AcrB. Here, we present the structure of E. coli AcrB in complex with deoxycholate at 3.85 resolution. All evidence suggests that bile acid is transported out of the cell via the periplasmic vestibule of the AcrAB-TolC complex.

引用

页码：677 / 682

页数：6

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