Nephroblastoma overexpressed gene (NOV) codes for a growth factor that induces protein tyrosine phosphorylation

被引：49

作者：

Liu, CL ^{[1
]}

Liu, XJ ^{[1
]}

Crowe, PD ^{[1
]}

Kelner, GS ^{[1
]}

Fan, J ^{[1
]}

Barry, G ^{[1
]}

Manu, F ^{[1
]}

Ling, N ^{[1
]}

De Souza, EB ^{[1
]}

Maki, RA ^{[1
]}

机构：

[1] Neurocrine Biosci Inc, San Diego, CA 92121 USA

来源：

GENE | 1999年 / 238卷 / 02期

关键词：

dexamethasone regulation; rat cDNA;

D O I：

10.1016/S0378-1119(99)00364-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

NOV (nephroblastoma overexpressed gene) is a member of the CCN (connective tissue growth factor [CTGF], Cyr61/Cef10, NOV) family of proteins. These proteins are cysteine-rich and are noted for having growth-regulatory functions. We have isolated the rat NOV gene, and the DNA sequence shares 90% identity with the mouse and 80% identity with the human sequences. The rat NOV gene was expressed in all rat tissues examined, including brain, lung, heart, kidney, liver, spleen, thymus and skeletal muscle. Higher levels of rat NOV mRNA were seen in the brain, lung and skeletal muscle compared to the other tissues. Examination of NOV expression in various human cell lines revealed that NOV was expressed in U87, 293, T98G, SK-N-MC and Hs683 but not in HepG2, HL60, THP1 and Jurkat. The human NOV gene was transfected into 293 cells and the expressed protein purified. When 3T3 fibroblasts were treated with this recombinant NOV protein, a dose-dependent increase in proliferation was observed. Analysis of tyrosine-phosphorylated proteins revealed that when 3T3 cells were treated with NOV, a 221 kDa protein was phosphorylated. These data suggest that NOV can act as a growth factor for some cells and binds to a specific receptor that leads to the phosphorylation of a 221 kDa protein. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：471 / 478

页数：8

共 28 条

[1] THE MODULAR ARCHITECTURE OF A NEW FAMILY OF GROWTH-REGULATORS RELATED TO CONNECTIVE-TISSUE GROWTH-FACTOR [J].