Disruption of the mitotic kinesin Eg5 gene (Knsl1) results in early embryonic lethality

被引：37

作者：

Chauviere, Muriel ^{[1
]}

Kress, Chantal ^{[2
]}

Kress, Michel ^{[1
]}

机构：

[1] Inst Andre Lwoff, CNRS, FRE Genet Mol & Integrat Fonct Cellulaires 2937, F-94801 Villejuif, France

[2] Inst Pasteur, CNRS, URA 2578, F-75724 Paris 15, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 04期

关键词：

mitotic kinesin-5; gene targeting; knockout; mouse preimplantation lethality;

D O I：

10.1016/j.bbrc.2008.04.177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eg5, a member of the widely conserved kinesin-5 family, is a plus-end-directed motor involved in separation of centrosomes, and in bipolar spindle formation and maintenance during mitosis in vertebrates. To investigate the requirement for Eg5 in mammalian development, we have generated Eg5 deficient mice by gene targeting. Heterozygous mice are healthy, fertile, and show no detectable phenotype, whereas Eg5(-/-) embryos die during early embryogenesis, prior to the implantation stage. This result shows that Eg5 is essential during early mouse development and cannot be compensated by another molecular motor. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：513 / 519

页数：7

共 29 条

[1] Phosphorylation by p34(cdc2) regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo [J].

Blangy, A ;

Lane, HA ;

dHerin, P ;

Harper, M ;

Kress, M ;

Nigg, EA .

CELL, 1995, 83 (07) :1159-1169

[2]

Blangy A, 1998, CELL MOTIL CYTOSKEL, V40, P174, DOI 10.1002/(SICI)1097-0169(1998)40:2<174::AID-CM6>3.0.CO

[3]

2-F

[4] The kinesin related motor protein, Eg5, is essential for maintenance of pre-implantation embryogenesis [J].

Castillo, Andrew ;

Justice, Monica J. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 357 (03) :694-699

[5] The Knsl1 gene encoding the kinesin-related protein MmEg5 maps to mouse chromosome 19 and a related pseudogene maps to mouse chromosome 6 [J].

Chauvière, M ;

Poirier, C ;

d'Hérin, P ;

Kress, M .

CYTOGENETICS AND CELL GENETICS, 2000, 89 (1-2) :16-17

[6] MUTATION OF A GENE THAT ENCODES A KINESIN-LIKE PROTEIN BLOCKS NUCLEAR DIVISION IN ASPERGILLUS-NIDULANS [J].

ENOS, AP ;

MORRIS, NR .

CELL, 1990, 60 (06) :1019-1027

[7]

Ferhat L, 1998, J NEUROSCI, V18, P7822

[8] NOVEL POTENTIAL MITOTIC MOTOR PROTEIN ENCODED BY THE FISSION YEAST CUT7+ GENE [J].

HAGAN, I ;

YANAGIDA, M .

NATURE, 1990, 347 (6293) :563-566

[9] THE KINESIN-LIKE PROTEIN KLP61F IS ESSENTIAL FOR MITOSIS IN DROSOPHILA [J].

HECK, MMS ;

PEREIRA, A ;

PESAVENTO, P ;

YANNONI, Y ;

SPRADLING, AC ;

GOLDSTEIN, LSB .

JOURNAL OF CELL BIOLOGY, 1993, 123 (03) :665-679

[10] 2 SACCHAROMYCES-CEREVISIAE KINESIN-RELATED GENE-PRODUCTS REQUIRED FOR MITOTIC SPINDLE ASSEMBLY [J].

HOYT, MA ;

HE, L ;

LOO, KK ;

SAUNDERS, WS .

JOURNAL OF CELL BIOLOGY, 1992, 118 (01) :109-120

← 1 2 3 →