Urea-formaldehyde resin monolith as a new packing material for affinity chromatography

被引：30

作者：

Sun, XF ^{[1
]}

Chai, ZK ^{[1
]}

机构：

[1] Acad Sinica, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期

基金：

中国国家自然科学基金;

关键词：

urea-formaldehyde resin monolithic column; monolithic column; stationary phases; LC; affinity adsorbents; Cibacron blue F3GA; proteins;

D O I：

10.1016/S0021-9673(01)01478-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A urea-formaldehyde resin (UF) continuous bed has been prepared through in-situ condensation polymerization in a confined tube. The monolith is an agglomerate of 2-mum irregular particles. Nitrogen adsorption shows that the monolith has a bimodal pore size distribution. It has low resistance to flow. A dyed monolith is obtained through modification of the UF monolith with Cibacron blue F3GA. Although its dye concentration and dynamic capacity axe low compared to Sepharose type affinity media, the dyed monolith can separate some proteins in the affinity mode of liquid chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：209 / 218

页数：10

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