Genome characterization of a novel porcine bocavirus

被引：72

作者：

Yang, Wan-zhu ^{[1
]}

Yu, Jie-mei ^{[1
]}

Li, Jin-song ^{[1
]}

Cheng, Wei-xia ^{[1
]}

Huang, Can-ping ^{[1
]}

Duan, Zhao-jun ^{[1
]}

机构：

[1] China Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Dept Viral Diarrhea, Beijing 100052, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 11期

关键词：

ADENOASSOCIATED VIRUS TYPE-2; HUMAN PARVOVIRUS B19; MINUTE VIRUS; GENETIC DIVERSITY; CAPSID PROTEIN; IDENTIFICATION; RECOMBINATION; EVOLUTION; MICE; INFECTIVITY;

D O I：

10.1007/s00705-012-1407-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Using a high-throughput DNA sequencing method, one DNA sequence (contig01006), suspected to belong to a novel porcine bocavirus (PBoV), was found with a high rate of detection (19.6 %) in fecal samples from healthy piglets. Moreover, a novel PBoV (tentatively named PBoV3C) with a nearly complete genome sequence (5235 bp) was identified. PBoV3C exhibits typical genome characteristics of bocaviruses and shows the highest genomic sequence identity (78 % to 81 %) to PBoV3A/B (PBoV3/4-UK) and PBoV3D/E (PBoV3/4-HK), respectively. Phylogenetic and recombination analysis indicated high diversity, prevalence and complexity among the PBoVs. The phospholipase A2 (PLA2) site of VP1 and the secondary structure of VP2 of PBoV3C were also analyzed. Additionally, we propose a uniform method of PBoV nomenclature based on the VP1 gene.

引用

页码：2125 / 2132

页数：8

共 46 条

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